Recombinant Anti-PSPH Antibody, Rabbit Monoclonal

EliteRmab®, manufactured by recombinant technology

Recombinant Anti-PSPH Antibody, Rabbit Monoclonal の製品情報

製品名
Recombinant Anti-PSPH Antibody, Rabbit Monoclonal
検証済のアプリケーション
WB,ELISA,ICC/IF,IP
交差反応
Reacts with: Human
特異性
Human PSPH
免疫原
Recombinant Human PSPH protein (Catalog#13503-HNAE)
調製
This antibody was obtained from a rabbit immunized with purified, recombinant Human PSPH (rh PSPH; Catalog#13503-HNAE; P78330; Met1-Glu225).
ソース
Monoclonal Rabbit IgG Clone #001
精製
Protein A
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-PSPH Antibody, Rabbit Monoclonal 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:500-1:1000
ELISA 1:25000-1:50000
ICC-IF 1:20-1:100
IP 0.2-1 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-PSPH Antibody, Rabbit Monoclonal の画像

Anti-PSPH rabbit monoclonal antibody at 1:500 dilution

Lane A: MCF7 Whole Cell Lysate

Lane B: K562 Whole Cell Lysate

Lane C: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:25 kDa

Observed band size:27 kDa

PSPH was immunoprecipitated using:

Lane A:0.5 mg K562 Whole Cell Lysate

Lane B:0.5 mg HepG2 Whole Cell Lysate

0.5 µL anti-PSPH rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-PSPH rabbit monoclonal antibody,at 1:1000 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 25 kDa

Observed band size: 25 kDa

Immunofluorescence staining of Human PSPH in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PSPH monoclonal antibody (dilution ratio 1:60) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue).

Recombinant Anti-PSPH Antibody, Rabbit Monoclonal: 別名

Anti-PSP Antibody; Anti-PSPHD Antibody

PSPH 背景情報

Phosphoserine phosphatase (PSPH) belongs to a subfamily of the phosphotransferases. PSPH is the rate-limiting enzyme in l-serine biosynthesis. It has previously been found that Phosphoserine phosphatase (PSPH) plays a role in epidermal homeostasis. Phosphoserine phosphatase (PSP) catalyzes the hydrolysis of phosphoserine to serine. Phosphoserine phosphatase (PSPH) expression has been examined in human-mouse somatic cell hybrids retaining different combination of human chromosomes. Phosphoserine phosphatase (PSPH) is expressed throughout the proliferative layer of the epidermis and hair follicles in rodent and human skin and is highly induced in SCC. In keratinocytes, Phosphoserine phosphatase (PSPH) is a cytoplasmic protein that primarily localizes to endosomes and is present primarily as a homodimer. Knock down of Phosphoserine phosphatase (PSPH) dramatically diminished SCC cell proliferation and cyclin D1 levels in the presence of exogenous of l-serine production suggesting a non-canonical role for Phosphoserine phosphatase (PSPH) in epithelial carcinogenesis. Phosphoserine phosphatase (PSPH) is highly induced in proliferative normal keratinocytes and skin tumors. Phosphoserine phosphatase (PSPH) appears to be critical for the proliferation of SCC cells; however, this phenomenon may not involve the phosphoserine metabolic pathway.
完全な名称
phosphoserine phosphatase
参考文献
  • Bachelor MA, et al. (2011) L-3-Phosphoserine phosphatase (PSPH) regulates cutaneous squamous cell carcinoma proliferation independent of L-serine biosynthesis. J Dermatol Sci. 63(3): 164-72.
  • Koch GA, et al. (1983) Assignment of the human phosphoserine phosphatase gene (PSP) to the pter leads to q22 region of chromosome 7. Cytogenet Cell Genet. 35(1): 67-9.
  • Jaeken J, et al. (1997) Phosphoserine phosphatase deficiency in a patient with Williams syndrome. J Med Genet. 34(7): 594-6.

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