Recombinant Anti-N Cadherin Antibody, Rabbit Monoclonal

RRID Number: AB_2860274
EliteRmab®, manufactured by recombinant technology
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Recombinant Anti-N Cadherin Antibody, Rabbit Monoclonal の製品情報

製品名
Recombinant Anti-N Cadherin Antibody, Rabbit Monoclonal
検証済のアプリケーション
WB,ELISA,FCM,ICC/IF
交差反応
Reacts with: Human
特異性
Human N Cadherin
免疫原
Recombinant Human N-Cadherin / CD325 / CDH2 Protein (Catalog#11039-H08H)
調製
This antibody was obtained from a rabbit immunized with purified, recombinant Human N-Cadherin / CD325 / CDH2 (rh N-Cadherin / CD325 / CDH2; Catalog#11039-H08H; NP_001783.2; Met 1-Ala 724).
ソース
Monoclonal Rabbit IgG Clone #020
精製
Protein A
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-N Cadherin Antibody, Rabbit Monoclonal 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:500-1:2000
ELISA 1:5000-1:10000
ICC-IF 1:20-1:100
FCM 1:25-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-N Cadherin Antibody, Rabbit Monoclonal の画像

Immunofluorescence staining of Human CDH2 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human CDH2 monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to membrane.
Flow cytometry analysis of N-Cadherin on HeLa cells. HeLa cells were harvested with (Left) or without (Right) trypsinization [please note, the epitope is sensitive to trypsin] and stained with either purified Rabbit IgG isotype control (dashed line) or Rabbit Anti-Human CD325 antibody [solid line, and Clone R001 (upper), Clone R020 (bottom)] at matching concentrations , then a FITC-conjugated second step antibody.

Anti-CDH2 rabbit monoclonal antibody at 1:500 dilution

Lane A: Hela Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:99 kDa

Observed band size:130 kDa

Citation List

Recombinant Anti-N Cadherin Antibody, Rabbit Monoclonal: 別名

Anti-CD325 Antibody; Anti-CDHN Antibody; Anti-CDw325 Antibody; Anti-NCAD Antibody

N Cadherin 背景情報

Cadherins are calcium dependent cell adhesion proteins, and they preferentially interact with themselves in a homophilic manner in connecting cells. Cadherin 2 (CDH2), also known as N-Cadherin (neuronal) (NCAD), is a single-pass tranmembrane protein and a cadherin containing 5 cadherin domains. N-Cadherin displays a ubiquitous expression pattern but with different expression levels between endocrine cell types. CDH2 (NCAD) has been shown to play an essential role in normal neuronal development, which is implicated in an array of processes including neuronal differentiation and migration, and axon growth and fasciculation. In addition, N-Cadherin expression was upregulated in human HSC during activation in culture, and function or expression blocking of N-Cadherin promoted apoptosis. During apoptosis, N-Cadherin was cleaved into 2-1 kDa fragments. It may provide a novel target for therapies that are directed toward intimal proliferative disorders, including restenosis and vascular bypass graft failure. N-Cadherin is associated with tumor aggressiveness and metastatic potential and may contribute to tumor progression.
完全な名称
cadherin 2, type 1, N-cadherin (neuronal)
Related Pathways
  • Canonical Wnt Pathway
    Canonical Wnt Pathway
参考文献
  • Jones M, et al. (2002) N-cadherin upregulation and function in response of smooth muscle cells to arterial injury. Arterioscler Thromb Vasc Biol. 22(12): 1972-7.
  • Nagi C, et al. (2005) N-cadherin expression in breast cancer: correlation with an aggressive histologic variant--invasive micropapillary carcinoma. Breast Cancer Res Treat. 94(3): 225-35.
  • Schrick C, et al. (2007) N-cadherin regulates cytoskeletally associated IQGAP1/ERK signaling and memory formation. Neuron. 55(5): 786-98.
  • Li K, et al. (2010) Downregulation of N-cadherin expression inhibits invasiveness, arrests cell cycle and induces cell apoptosis in esophageal squamous cell carcinoma. Cancer Invest. 28(5): 479-86.

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