Anti-kynureninase/KYNU Antibody, Rabbit Polyclonal

Anti-kynureninase/KYNU Antibody, Rabbit Polyclonal の製品情報

製品名
Anti-kynureninase/KYNU Antibody, Rabbit Polyclonal
検証済のアプリケーション
WB,ELISA,IP
交差反応
Reacts with: Human
特異性
Human kynureninase/KYNU
免疫原
Recombinant Human KYNU protein (Catalog#10841-H08B)
調製
Produced in rabbits immunized with purified, recombinant Human KYNU (rh KYNU; Catalog#10841-H08B; Q16719; Met1-Asn465). KYNU specific IgG was purified by Human KYNU affinity chromatography.
ソース
Polyclonal Rabbit IgG
精製
Protein A & Antigen Affinity
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-kynureninase/KYNU Antibody, Rabbit Polyclonal 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:500-1:2000
ELISA 1:5000-1:10000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-kynureninase/KYNU Antibody, Rabbit Polyclonal の画像

KYNU was immunoprecipitated using:

Lane A:0.5 mg A549 Whole Cell Lysate

4 µL anti-KYNU rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-KYNU rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution

Developed using the DAB staining technique.

Performed under reducing conditions.

Predicted band size: 52 kDa

Observed band size: 52 kDa

Anti-KYNU rabbit polyclonal antibody at 1:500 dilution

Lane A: A549 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:52 kDa

Observed band size:52 kDa

(We are unsure as to the identity of these extra bands.)

Anti-kynureninase/KYNU Antibody, Rabbit Polyclonal: 別名

Anti-KYNUU Antibody

kynureninase/KYNU 背景情報

KYNU (Kynureninase) is a Protein Coding gene. KYNU is a pyridoxal-5'-phosphate (pyridoxal-P) dependent enzyme that catalyzes the cleavage of L-kynurenine and L-3-hydroxykynurenine into anthranilic and 3-hydroxyanthranilic acids, respectively. Genetic studies in the mouse and the human suggest that kynureninase activity may influence blood pressure and renal function. The gene coding kynureninase (KYNU) is also located on chromosome band 2q14-q23, where a linkage peak for essential hypertension was previously detected in the Chinese Han population. The results show that the rare KYNU variant Arg188Gln affects kynureninase activity and are consistent with the hypothesis that this mutation can predispose to essential hypertension. Diseases associated with KYNU include Hydroxykynureninuria and Vertebral, Cardiac, Renal, And Limb Defects Syndrome 2.
完全な名称
kynureninase
参考文献
  • Lima S, et al. (2007) Crystal structure of Homo sapiens kynureninase. Biochemistry. 46(10): 2735-44.
  • Momany C, et al. (2004) Three-dimensional structure of kynureninase from Pseudomonas fluorescens. Biochemistry. 43(5): 1193-203.
  • Toma S, et al. (1997) Cloning and recombinant expression of rat and human kynureninase. FEBS Lett. 408(1): 5-10.

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