Anti-JNK2 Antibody, Rabbit Polyclonal

Anti-JNK2 Antibody, Rabbit Polyclonal の製品情報

製品名
Anti-JNK2 Antibody, Rabbit Polyclonal
検証済のアプリケーション
ELISA,IP
交差反応
Reacts with: Human
特異性
Human JNK2
免疫原
Recombinant Human JNK2 proteimn (Catalog#10745-H08B)
調製
Produced in rabbits immunized with purified, recombinant Human JNK2 / MAPK9 (rh JNK2 / MAPK9; Catalog#10745-H08B; NP_002743.3; Met 1-Arg 424). JNK2 / MAPK9 specific IgG was purified by Human JNK2 / MAPK9 affinity chromatography.
ソース
Polyclonal Rabbit IgG
精製
Protein A & Antigen Affinity
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-JNK2 Antibody, Rabbit Polyclonal 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
ELISA 1:5000-1:10000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-JNK2 Antibody, Rabbit Polyclonal の画像

MAPK9 was immunoprecipitated using:

Lane A:0.5 mg A549 Whole Cell Lysate

Lane B:0.5 mg MCF-7 Whole Cell Lysate

2 µL anti-MAPK9 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-MAPK9 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 44 kDa

Observed band size: 44 kDa

Anti-JNK2 Antibody, Rabbit Polyclonal: 別名

Anti-JNK-55 Antibody; Anti-JNK2 Antibody; Anti-JNK2A Antibody; Anti-JNK2ALPHA Antibody; Anti-JNK2B Antibody; Anti-JNK2BETA Antibody; Anti-p54a Antibody; Anti-p54aSAPK Antibody; Anti-PRKM9 Antibody; Anti-SAPK Antibody; Anti-SAPK1a Antibody

JNK2 背景情報

Mitogen-activated protein kinase 9 (MAPK9), also well known as c-Jun N-terminal kinase (JNK2), is a member of the MAP kinase subfamily belonging to the protein kinase superfamily. MAPK9 responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating some transcription factors, such as c-Jun and ATF2. The crystal structure of human JNK2 complexed with an indazole inhibitor by applying a high-throughput protein engineering and surface-site mutagenesis approach. A novel conformation of the activation loop is observed, which is not compatible with its phosphorylation by upstream kinases. This activation inhibitory conformation of JNK2 is stabilized by the MAP kinase insert that interacts with the activation loop in an induced-fit manner. It suggests that the MAP kinase insert of JNK2 plays a role in the regulation of JNK2 activation, possibly by interacting with intracellular binding partners. JNK2 deficiency leads to reduced c-Jun degradation, thereby augmenting c-Jun levels and cellular proliferation, and suggests that JNK2 is a negative regulator of cellular proliferation in multiple cell types. JNK2 prevents replicative stress by coordinating cell cycle progression and DNA damage repair mechanisms. JNK2 blocks the ubiquitination of tumor suppressor p53, and thus increases the stability of p53 in nonstressed cells. JNK2 negatively regulates antigen-specific CD8+ T cell expansion and effector function, and thus selectively blocking JNK2 in CD8+ T cells may potentially enhance the anti-tumor immune response. Lack of JNK2 expression was associated with higher tumor aneuploidy and reduced DNA damage response. Additionally, the JNK2 protein could be a novel therapeutic target in dry eye disease and may provide a novel target for the prevention of vascular disease and atherosclerosis.
完全な名称
mitogen-activated protein kinase 9
Related Pathways
  • VEGF Signaling Pathway
    VEGF Signaling Pathway
  • B Cell Receptor Signaling Pathway
    B Cell Receptor Signaling Pathway
  • T Cell Receptor Signaling Pathway
    T Cell Receptor Signaling Pathway
  • Autophagy Pathway
    Autophagy Pathway
  • IL1 signaling pathway
    IL1 signaling pathway
  • IL17 signaling pathway
    IL17 signaling pathway
参考文献
  • Sabapathy K, et al. (2004) JNK2: a negative regulator of cellular proliferation. Cell Cycle. 3(12): 1520-3.
  • Tao J, et al. (2007) JNK2 negatively regulates CD8+ T cell effector function and anti-tumor immune response. Eur J Immunol. 37(3): 818-29.
  • Shaw D, et al. (2008) The crystal structure of JNK2 reveals conformational flexibility in the MAP kinase insert and indicates its involvement in the regulation of catalytic activity. J Mol Biol. 383(4): 885-93.
  • Osto E, et al. (2008) c-Jun N-terminal kinase 2 deficiency protects against hypercholesterolemia-induced endothelial dysfunction and oxidative stress. 118(20): 2073-80.
  • De Paiva CS, et al. (2009) Essential role for c-Jun N-terminal kinase 2 in corneal epithelial response to desiccating stress. Arch Ophthalmol. 127(12): 1625-31.
  • Chen P, et al. (2010) Jnk2 effects on tumor development, genetic instability and replicative stress in an oncogene-driven mouse mammary tumor model. PLoS One. 5(5): e10443.

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