Anti-IL-32 Antibody, Mouse Monoclonal

Anti-IL-32 Antibody, Mouse Monoclonal の製品情報

製品名
Anti-IL-32 Antibody, Mouse Monoclonal
検証済のアプリケーション
WB
交差反応
Reacts with: Human
特異性
Human IL-32
免疫原
Recombinant Human IL-32 alpha / IL32A Protein (Catalog#11064-H08H)
調製
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IL-32 alpha / IL32A (rh IL-32 alpha / IL32A; Catalog#11064-H08H; NP_001012651.1; Met1-Lys131). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
ソース
Monoclonal Mouse IgG2a Clone #12
精製
Protein A
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-IL-32 Antibody, Mouse Monoclonal 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:500-1:1000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-IL-32 Antibody, Mouse Monoclonal の画像

Anti-IL32A mouse monoclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:26 kDa

Observed band size:30 kDa

(We are unsure as to the identity of these extra bands.)

Anti-IL-32 Antibody, Mouse Monoclonal: 別名

Anti-IL-32alpha Antibody; Anti-IL-32beta Antibody; Anti-IL-32delta Antibody; Anti-IL-32gamma Antibody; Anti-IL32 Antibody; Anti-NK4 Antibody; Anti-TAIF Antibody; Anti-TAIFa Antibody; Anti-TAIFb Antibody; Anti-TAIFc Antibody; Anti-TAIFd Antibody

IL-32 背景情報

IL-32 is a recently discovered cytokine that induces various proinflammatory cytokines (TNF-alpha, IL-1beta, IL-6) and chemokines in both human and mouse cells through the NF-kappaB and p38 MAPK inflammatory signal pathways. It is regulated robustly by other major proinflammatory cytokines and is crucial to inflammation and immune responses. Four of the IL-32 isoforms (alpha, beta, gamma, and delta) are the most representative IL-32 transcripts, and the gamma isoform of IL-32 is the most active, although all isoforms are biologically active. IL-32, a cytokine produced mainly by T, natural killer, and epithelial cells induces significant amounts of TNFalpha and MIP-2 and increases the production of both cytokines in a dose-dependent manner. IL-32 has been implicated in inflammatory disorders, Mycobacterium tuberculosis infections, inflammatory bowel disease, and influenza A virus infection, as well as in some autoimmune diseases, such as rheumatoid arthritis, ulcerative colitis, and in the human stomach cancer, human lung cancer, and breast cancer tissues. Thus, IL-32 expression might be valuable as a biomarker for cancer.
完全な名称
interleukin 32
参考文献
  • Felaco P, et al. (2009) IL-32: a newly-discovered proinflammatory cytokine. J Biol Regul Homeost Agents. 23(3): 141-7.
  • Kobayashi H, et al. (2009) Molecular characterization of IL-32 in human endothelial cells. Cytokine. 46(3): 351-8.
  • Meyer N, et al. (2010) IL-32 is expressed by human primary keratinocytes and modulates keratinocyte apoptosis in atopic dermatitis. J Allergy Clin Immunol. 125(4): 858-865.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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