Anti-HNMT Antibody, Rabbit Polyclonal

Anti-HNMT Antibody, Rabbit Polyclonal の製品情報

製品名
Anti-HNMT Antibody, Rabbit Polyclonal
検証済のアプリケーション
WB,ELISA,IP
交差反応
Reacts with: Human
特異性
Human HNMT
免疫原
Recombinant Human HNMT protein (Catalog#13096-H09E)
調製
Produced in rabbits immunized with purified, recombinant Human HNMT (rh HNMT; Catalog#13096-H09E; AAH20677.1; Met 1-Ala 292). HNMT specific IgG was purified by Human HNMT affinity chromatography.
ソース
Polyclonal Rabbit IgG
精製
Protein A & Antigen Affinity
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-HNMT Antibody, Rabbit Polyclonal 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:500-1:2000
ELISA 1:5000-1:10000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-HNMT Antibody, Rabbit Polyclonal の画像

Anti-HNMT rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:33 kDa

Observed band size:32 kDa

(We are unsure as to the identity of these extra bands.)

HNMT was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

Lane B:0.5 mg Hela Whole Cell Lysate

2 µL anti-HNMT rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-HNMT rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 33 kDa

Observed band size: 33 kDa

Anti-HNMT Antibody, Rabbit Polyclonal: 別名

Anti-HMT Antibody; Anti-HNMT-S1 Antibody; Anti-HNMT-S2 Antibody

HNMT 背景情報

HNMT (Histamine N-methyltransferase) is a Protein Coding gene. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. HNMT, the major enzyme for the metabolism of histamine in the rat brain, is potently inhibited by 9-amino-1,2,3,4-tetrahydroacridine (tacrine). Methylation is an important pathway in the biotransformation of many drugs, neurotransmitters, and xenobiotic compounds. Histamine N-methyltransferase (HNMT) catalyzes the N tau-methylation of histamine and structurally related compounds. Histamine N-methyltransferase (HNMT) is believed to be the sole pathway for termination of the neurotransmitter action of histamine in the mammalian brain. That highlights the importance of the inclusion of HNMT for genetic testing of individuals presenting with intellectual disability.
完全な名称
histamine N-methyltransferase
参考文献
  • Yan L., et al., 2000, Pharmacogenetics 10:261-266.
  • Horton J.R., et al., 2001, Structure 9:837-849.
  • Hillier L.W., et al., 2005, Nature 434:724-731.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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