Anti-Galectin 3/LGALS3 Antibody, Rabbit Polyclonal

Anti-Galectin 3/LGALS3 Antibody, Rabbit Polyclonal の製品情報

製品名
Anti-Galectin 3/LGALS3 Antibody, Rabbit Polyclonal
検証済のアプリケーション
WB,IP
交差反応
Reacts with: Human
特異性
Human Galectin 3/LGALS3
免疫原
Recombinant Human LGALS3 / Galectin-3 protein (Catalog#10289-HNAE)
調製
Produced in rabbits immunized with purified, recombinant Human LGALS3 / Galectin-3 (rh LGALS3 / Galectin-3; Catalog#10289-HNAE; P17931; Met1-Ile250). LGALS3 / Galectin-3 specific IgG was purified by Human LGALS3 / Galectin-3 affinity chromatography.
ソース
Polyclonal Rabbit IgG
精製
Protein A & Antigen Affinity
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Galectin 3/LGALS3 Antibody, Rabbit Polyclonal 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:1000-1:5000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Galectin 3/LGALS3 Antibody, Rabbit Polyclonal の画像

Anti-Galectin3 rabbit polyclonal antibody at 1:1000 dilution

Lane A: MCF7 Whole Cell Lysate

Lane B: Hela Whole Cell Lysate

Lysates/proteins at 20 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:26 kDa

Observed band size:30 kDa

Galectin3 was immunoprecipitated using:

Lane A:0.5 mg MCF-7 Whole Cell Lysate

Lane B:0.5 mg Hela Whole Cell Lysate

Lane C:0.5 mg SW480 Whole Cell Lysate

Lane D:0.5 mg A431 Whole Cell Lysate

2 µL anti-Galectin3 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-Galectin3 rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 30 kDa

Observed band size: 30 kDa

Anti-Galectin 3/LGALS3 Antibody, Rabbit Polyclonal: 別名

Anti-CBP35 Antibody; Anti-GAL3 Antibody; Anti-GALBP Antibody; Anti-GALIG Antibody; Anti-L31 Antibody; Anti-LGALS2 Antibody; Anti-MAC2 Antibody

Galectin 3/LGALS3 背景情報

LGALS3 (Galectin 3) is a Protein Coding gene. This gene encodes a member of the galectin family of carbohydrate-binding proteins. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. LGALS3 is a beta-galactoside-binding lectin and plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion, and T-cell regulation. LGALS3 has an important role in tumor progression through inhibition of apoptosis. LGALS3 expression is associated with neoplastic transformation and with differentiation of monocytes to macrophages. Elevated expression of LGALS3 has been demonstrated in the synovium of rheumatoid arthritis (RA). Diseases associated with LGALS3 include Follicular Adenoma and Papillary Carcinoma.
完全な名称
lectin, galactoside-binding, soluble, 3
参考文献
  • Dumic J, et al. (2006) Galectin-3: an open-ended story. Biochim Biophys Acta. 1760(4): 616-35.
  • Sharma UC, et al. (2004) Galectin-3 marks activated macrophages in failure-prone hypertrophied hearts and contributes to cardiac dysfunction. Circulation. 110(19): 3121-8.
  • Yan YP, et al. (2009) Galectin-3 mediates post-ischemic tissue remodeling. Brain Res. 1288: 116-24.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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