Recombinant Anti-EpCAM Antibody, Rabbit Monoclonal

RRID Number: AB_2860214
EliteRmab®, manufactured by recombinant technology

Recombinant Anti-EpCAM Antibody, Rabbit Monoclonal の製品情報

製品名
Recombinant Anti-EpCAM Antibody, Rabbit Monoclonal
検証済のアプリケーション
WB,ELISA,ELISA(Det),IHC-P,FCM,ICC/IF,IP
交差反応
Reacts with: Human
特異性
Human EpCAM
No cross-reactivity in ELISA with
Human CD146 / MCAM
Human CD171 / N-CAML1 / L1CAM
Human OBCAM / OPCML
Human TROP2 / TACSTD2
免疫原
Recombinant Human EpCAM protein (Catalog#10694-H08H)
調製
This antibody was obtained from a rabbit immunized with purified, recombinant Human EpCAM / TROP1 (rh EpCAM; Catalog#10694-H08H; NP_002345.1; Met 1-Lys 265).
ソース
Monoclonal Rabbit IgG Clone #28
精製
Protein A
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-EpCAM Antibody, Rabbit Monoclonal 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:500-1:1000
ELISA 1:25000-1:50000
IHC-P 1:1000-1:5000
ICC-IF 1:250-1:5000
FCM 1:100-1:500
IP 0.2-1 μL/mg of lysate
ELISA(Det) 1:5000-1:50000
Notes
ELISA(Det): This antibody will detect Human EpCAM / TROP-1 / TACSTD1 in ELISA pair set (Catalog: # SEK10694). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 10694-MM07).
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-EpCAM Antibody, Rabbit Monoclonal の画像

Immunofluorescence staining of Human EpCAM in SKBR3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human EpCAM monoclonal antibody (1:500) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.
Immunochemical staining of human EpCAM in human mammary gland with rabbit monoclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to membrane of alveolus epithelium.
Immunochemical staining of human EpCAM in human colon carcinoma with rabbit monoclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to membrane of colonic gland epithelium.
Immunochemical staining of human EpCAM in human bladder carcinoma with rabbit monoclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to membrane of transitional epithelium.
Flow cytometric analysis of anti-EpCAM (CD326) reactivity on SKBR3 cells. SKBR3 cells were stained with anti-EpCAM (CD326) Monoclonal Ab (10694-R028), then a FITC-conjugated second step antibody. The histogram were derived from the gated events based on light scattering characteristics of viable cells.

Anti-EpCAM rabbit monoclonal antibody at 1:500 dilution

Lane A: MCF-7 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:34 kDa

Observed band size:38 kDa

EpCAM was immunoprecipitated using:

Lane A:0.5 mg MCF-7 Whole Cell Lysate

Lane B:0.5 mg A431 Whole Cell Lysate

Lane C:0.5 mg HepG2 Whole Cell Lysate

Lane D:0.5 mg Caco-2 Whole Cell Lysate

0.5 µL anti-EpCAM rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-EpCAM rabbit monoclonal antibody,at 1:5000 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 40 kDa

Observed band size: 40 kDa

Recombinant Anti-EpCAM Antibody, Rabbit Monoclonal: 別名

Anti-DIAR5 Antibody; Anti-EGP-2 Antibody; Anti-EGP314 Antibody; Anti-EGP40 Antibody; Anti-ESA Antibody; Anti-HNPCC8 Antibody; Anti-KS1/4 Antibody; Anti-KSA Antibody; Anti-M4S1 Antibody; Anti-MIC18 Antibody; Anti-MK-1 Antibody; Anti-TACSTD1 Antibody; Anti-TROP-1 Antibody; Anti-TROP1 Antibody

EpCAM 背景情報

Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type â…transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway.
完全な名称
epithelial cell adhesion molecule
参考文献
  • Brunner A, et al. (2008) EpCAM is predominantly expressed in high grade and advanced stage urothelial carcinoma of the bladder. J Clin Pathol. 61(3):307-10.
  • Trzpis M, et al. (2008) EpCAM in morphogenesis. Front Biosci. 13: 5050-5.
  • Munz M, et al. (2009) The emerging role of EpCAM in cancer and stem cell signaling. Cancer Res. 69(14): 5627-9.
  • Carpenter G, et al. (2009) EpCAM: another surface-to-nucleus missile. Cancer Cell. 15(3): 165-6.
  • HRP-conjugated plug-and-playable IgG-binding nanobodies as secondary antibody mimics in immunoassays
    Author
    Bae, Y;Jang, D;Eom, S;Park, T;Kang, S;
    Year
    2020
    Journal
    Sensors and Actuators B: Chemical
  • Development of an Improved 3D in vitro Intestinal Model to Perform Permeability Studies of Paracellular Compounds
    Author
    Macedo, M;Martínez, E;Barrias, C;Sarmento, B;
    Year
    2020
    Journal
    Front. Bioeng. Biotechnol.
    Application
    IHC

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