Anti-E-Cadherin Antibody, Mouse Monoclonal

Anti-E-Cadherin Antibody, Mouse Monoclonal の製品情報

製品名
Anti-E-Cadherin Antibody, Mouse Monoclonal
検証済のアプリケーション
ELISA(Cap),ICC/IF
交差反応
Reacts with: Human
特異性
Human E-Cadherin
免疫原
Recombinant Human Cadherin-1 / CDH1 / CD324 / E-cadherin protein (Catalog#10204-H08H)
調製
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Cadherin-1 / CDH1 / CD324 / E-cadherin (rh Cadherin-1 / CDH1 / CD324 / E-cadherin; Catalog#10204-H08H; NP_004351.1; Met 1-Ile 707). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
ソース
Monoclonal Mouse IgG1 Clone #7G5D4
精製
Protein A
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-E-Cadherin Antibody, Mouse Monoclonal 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
ICC-IF 1:20-1:200
ELISA(Cap) 1:250-1:2000
Notes
ELISA(Cap): This antibody will detect Human Cadherin-1 / CDH1 / CD324 / E-cadherin in ELISA pair set (Catalog: # SEK10204). In a sandwich ELISA, it can be used as capture antibody.
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-E-Cadherin Antibody, Mouse Monoclonal の画像

Immunofluorescence staining of human E-cadherin in MCF-7 cells with mouse monoclonal antibody (dilution ratio 1:60). The image showing membrane staining of MCF-7 cells.

Anti-E-Cadherin Antibody, Mouse Monoclonal: 別名

Anti-Arc-1 Antibody; Anti-CD324 Antibody; Anti-CDH1 Antibody; Anti-CDHE Antibody; Anti-E-cad Antibody; Anti-E-Cadherin Antibody; Anti-ECAD Antibody; Anti-LCAM Antibody; Anti-UVO Antibody

E-Cadherin 背景情報

Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is a calcium-dependent cell adhesion molecule the intact function of which is crucial for the establishment and maintenance of epithelial tissue polarity and structural integrity. Mutations in CDH1 occur in diffuse type gastric cancer, lobular breast cancer, and endometrial cancer. In human cancers, partial or complete loss of E-cadherin expression correlates with malignancy. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas.
完全な名称
cadherin 1, type 1, E-cadherin (epithelial)
Related Pathways
  • Canonical Wnt Pathway
    Canonical Wnt Pathway
  • EGFR Signaling Pathway
    EGFR Signaling Pathway
参考文献
  • Wang HD, et al. (2004) CDH1 germline mutation in hereditary gastric carcinoma. World J Gastroenterol. 10(21): 3088-93.
  • Masterson J, et al. (2007) Posttranslational truncation of E-cadherin and significance for tumour progression. Cells Tissues Organs. 185(1-3): 175-9.
  • Mrgineanu E, et al. (2008) Correlation between E-cadherin abnormal expressions in different types of cancer and the process of metastasis. Rev Med Chir Soc Med Nat Iasi. 112(2): 432-6.

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Mouse MAb

Rabbit PAb

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