Anti-CD73 Antibody, Mouse Monoclonal

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Anti-CD73 Antibody, Mouse Monoclonal の製品情報

製品名
Anti-CD73 Antibody, Mouse Monoclonal
検証済のアプリケーション
FCM
交差反応
Reacts with: Human
特異性
Human CD73
免疫原
Recombinant Human CD73 / NT5E Protein (Catalog#10904-H08H)
調製
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD73 / NT5E (rh CD73 / NT5E; Catalog#10904-H08H; NP_002517.1; Met1-Lys547). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
ソース
Monoclonal Mouse IgG1 Clone #07
精製
Protein A
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CD73 Antibody, Mouse Monoclonal 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
FCM 1:25-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-CD73 Antibody, Mouse Monoclonal の画像

Flow cytometric analysis of Human NT5E(CD73) expression on human whole blood lymphocytes. Cells were stained with purified anti-Human NT5E(CD73), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.

Anti-CD73 Antibody, Mouse Monoclonal: 別名

Anti-CALJA Antibody; Anti-CD73 Antibody; Anti-E5NT Antibody; Anti-eN Antibody; Anti-eNT Antibody; Anti-NT Antibody; Anti-NT5 Antibody; Anti-NTE Antibody

CD73 背景情報

5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein that belongs to the 5'-nucleotidase family. CD73 is a glycosylphosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signaling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1, and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purinergic receptors to induce cellular responses. CD73 has also been reported to regulate the expression of pro-inflammatory molecules in mouse endothelium.
完全な名称
5'-nucleotidase, ecto (CD73)
参考文献
  • Resta R. et al., 1997, Cell Signal. 9 (2): 131-9.
  • Yamashita Y. et al., 1998, Eur J Immunol. 28 (10): 2981-90.
  • Louis NA. et al., 2008, J Immunol. 180 (6): 4246-55.
  • Grünewald JK. et al., 2010, J Inflamm. 7 (1): 10.

Standard Antibody Development Service

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Mouse MAb

Rabbit PAb

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