Recombinant Anti-c-Kit Antibody, Rabbit Monoclonal

EliteRmab®, manufactured by recombinant technology

Recombinant Anti-c-Kit Antibody, Rabbit Monoclonal の製品情報

製品名
Recombinant Anti-c-Kit Antibody, Rabbit Monoclonal
検証済のアプリケーション
ELISA,IHC-P
交差反応
Reacts with: Human
特異性
Human c-Kit
No cross-reactivity in ELISA with
Mouse KIT
Human PDGFRa
Human PDGFRb
免疫原
Recombinant Human c-Kit / CD117 protein (Catalog#11996-H08H)
調製
This antibody was obtained from a rabbit immunized with purified, recombinant Human c-Kit / CD117 (rh c-Kit / CD117; Catalog#11996-H08H; P10721-2; Met1-Thr516).
ソース
Monoclonal Rabbit IgG Clone #351
精製
Protein A
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-c-Kit Antibody, Rabbit Monoclonal 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
ELISA 1:5000-1:10000
IHC-P 1:100-1:500
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-c-Kit Antibody, Rabbit Monoclonal の画像

Immunochemical staining of human c-KIT in human skin with rabbit monoclonal antibody (1:300, formalin-fixed paraffin embedded sections).
Immunochemical staining of human c-KIT in human melanoma with rabbit monoclonal antibody (1:300, formalin-fixed paraffin embedded sections).
Immunochemical staining of human c-KIT in human breast cancer with rabbit monoclonal antibody (1:300, formalin-fixed paraffin embedded sections).

Recombinant Anti-c-Kit Antibody, Rabbit Monoclonal: 別名

Anti-C-Kit Antibody; Anti-CD117 Antibody; Anti-PBT Antibody; Anti-SCFR Antibody

c-Kit 背景情報

C-Kit is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). c-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains and 1 protein kinase domain. It belongs to the protein kinase superfamily, tyr protein kinase family, and CSF-1/PDGF receptor subfamily. C-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains and 1 protein kinase domain. C-Kit has tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase. Antibodies to c-Kit are widely used in immunohistochemistry to help distinguish particular types of tumor in histological tissue sections. It is used primarily in the diagnosis of GISTs. In GISTs, c-Kit staining is typically cytoplasmic, with stronger accentuation along the cell membranes. C-Kit antibodies can also be used in the diagnosis of mast cell tumors and in distinguishing seminomas from embryonal carcinomas. Mutations in the c-Kit gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Defects in KIT are a cause of acute myelogenous leukemia (AML). AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development. Note=Somatic mutations that lead to constitutive activation of KIT are detected in AML patients.
完全な名称
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
参考文献
  • Andre C, et al. (1997) Sequence analysis of two genomic regions containing the KIT and the FMS receptor tyrosine kinase genes. Genomics. 39(2):216-26.
  • Yarden Y, et al. (1987) Human proto-oncogene c-kit: a new cell surface receptor tyrosine kinase for an unidentified ligand. EMBO J. 6(11):3341-51.
  • Leong KG, et al. (2008) Generation of a prostate from a single adult stem cell. Nature. 456(7223): 804-8.
  • Edling CE, et al. (2007) c-Kit--a hematopoietic cell essential receptor tyrosine kinase. Int J Biochem Cell Biol. 39(11):1995-8.
  • McIntyre A, et al. (2005) Amplification and overexpression of the KIT gene is associated with progression in the seminoma subtype of testicular germ cell tumors of adolescents and adults. Cancer Res. 65(18):8085-9.
  • Protein expression of KIT, BRAF, GNA11, GNAQ and RASSF1 in feline diffuse iris melanomas
    Author
    Rushton, J;Korb, M;Kummer, S;Reichart, U;Fuchs-Baumgartinger, A;Tichy, A;Nell, B;
    Year
    2019
    Journal
    The Veterinary Journal
    Application
    IF

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

カートに追加しました カートに追加しませんでした カートを更新しています。少々お待ちください U.S.A.