Recombinant Anti-beta-Catenin Antibody, Rabbit Monoclonal

RRID Number: AB_2860341
EliteRmab®, manufactured by recombinant technology

Recombinant Anti-beta-Catenin Antibody, Rabbit Monoclonal の製品情報

製品名
Recombinant Anti-beta-Catenin Antibody, Rabbit Monoclonal
検証済のアプリケーション
ELISA,IHC-P,FCM,ICC/IF
交差反応
Reacts with: Human
特異性
Human beta-Catenin
No cross-reactivity in ELISA with
Mouse CTNNB1
免疫原
Recombinant Human beta-Catenin / CTNNB1 protein (Catalog#11279-H20B)
調製
This antibody was obtained from a rabbit immunized with purified, recombinant Human beta-Catenin / CTNNB1 (rh beta-Catenin / CTNNB1; Catalog#11279-H20B; P35222-1; Met1-Leu781).
ソース
Monoclonal Rabbit IgG Clone #021
精製
Protein A
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-beta-Catenin Antibody, Rabbit Monoclonal 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
ELISA 1:5000-1:10000
IHC-P 1:100-1:500
ICC-IF 1:20-1:100
FCM 1:25-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-beta-Catenin Antibody, Rabbit Monoclonal の画像

Immunofluorescence staining of Human CTNNB1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CTNNB1 monoclonal antibody (1:60) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.
Immunochemical staining of human CTNNB1 in human skin with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).
Immunochemical staining of human CTNNB1 in human colon with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).
Flow cytometric analysis of Human CTNNB1 (β-Catenin) expression on Hela cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human CTNNB1 (β-Catenin), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Recombinant Anti-beta-Catenin Antibody, Rabbit Monoclonal: 別名

Anti-armadillo Antibody; Anti-CTNNB Antibody; Anti-MRD19 Antibody

beta-Catenin 背景情報

beta-Catenin, also known as CTNNB1, is a member of the armadillo family of proteins. These proteins have multiple copies of the so-called armadillo repeat domain, which is specialized for protein-protein binding. It is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. CTNNB1 also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, beta-Catenin binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Defects in beta-Catenin can cause colorectal cancer, pilomatrixoma (PTR), medulloblastoma, and ovarian cancer. CTNNB1 is a key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, it forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, beta-Catenin is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. CTNNB1 is involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
完全な名称
catenin beta 1
Related Pathways
  • Canonical Wnt Pathway
    Canonical Wnt Pathway
  • EGFR Signaling Pathway
    EGFR Signaling Pathway
  • Non-Canonical Wnt Pathway
    Non-Canonical Wnt Pathway
参考文献
  • Yang, et al. (2002) Linking β-catenin to androgen-signaling pathway. J Biol Chem. 277(13):11336-44.
  • Hino S, et al. (2005) Phosphorylation of β-Catenin by Cyclic AMP-Dependent Protein Kinase Stabilizes β-Catenin through Inhibition of Its Ubiquitination. Mol Cell Biol. 25(20):9063-72.
  • Liu X, et al. (2005) Rapid, Wnt-induced changes in GSK3beta associations that regulate beta-catenin stabilization are mediated by Galpha proteins. Curr Biol. 15(22):1989-97.
  • Kraus C, et al. (1994) Localization of the human β-catenin gene (CTNNB1) to 3p21: a region implicated in tumor development. Genomics. 23(1):272-4.

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