Anti-14-3-3 eta/YWHAH Antibody, Rabbit Polyclonal

Anti-14-3-3 eta/YWHAH Antibody, Rabbit Polyclonal の製品情報

製品名
Anti-14-3-3 eta/YWHAH Antibody, Rabbit Polyclonal
検証済のアプリケーション
WB,ELISA,IP
交差反応
Reacts with: Human
特異性
Human 14-3-3 eta/YWHAH
免疫原
Recombinant Human 14-3-3 eta / YWHAH protein (Catalog#10847-H09E)
調製
Produced in rabbits immunized with purified, recombinant Human 14-3-3 eta / YWHAH (rh 14-3-3 eta / YWHAH; Catalog#10847-H09E; Q04917; Gly2-Asn246). 14-3-3 eta / YWHAH specific IgG was purified by Human 14-3-3 eta / YWHAH affinity chromatography.
ソース
Polyclonal Rabbit IgG
精製
Protein A & Antigen Affinity
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-14-3-3 eta/YWHAH Antibody, Rabbit Polyclonal 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:500-1:2000
ELISA 1:5000-1:10000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-14-3-3 eta/YWHAH Antibody, Rabbit Polyclonal の画像

Anti-YWHAH rabbit polyclonal antibody at 1:500 dilution

Lane A: U937 Whole Cell Lysate

Lane B: 293T Whole Cell Lysate

Lane C: Jurkat Whole Cell Lysate

Lane D: K562 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:33 kDa

Observed band size:31 kDa

YWHAH was immunoprecipitated using:

Lane A:0.5 mg 293T Whole Cell Lysate

Lane B:0.5 mg Jurkat Whole Cell Lysate

Lane C:0.5 mg k562 Whole Cell Lysate

Lane D:0.5 mg NIH-3T3 Whole Cell Lysate

2 µL anti-YWHAH rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-YWHAH rabbit polyclonal antibody,at 1:2000 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 28 kDa

Observed band size: 28 kDa

Anti-14-3-3 eta/YWHAH Antibody, Rabbit Polyclonal: 別名

Anti-YWHA1 Antibody

14-3-3 eta/YWHAH 背景情報

YWHAH, the gene encoding the 14-3-3eta isoform, is highly expressed in retinal ganglion cells (RGC). YWHAH is a positional and functional candidate gene for both schizophrenia and bipolar disorder (BP). It is located on chromosome 22q12.3, a region that has been implicated by linkage studies in both BP and schizophrenia.
完全な名称
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta
Related Pathways
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    Death Receptor Signaling
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    TNF Signaling
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    Actin Dynamics Signaling Pathway
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    AKT Signaling Pathway
  • MAPK-Erk Pathway
    MAPK-Erk Pathway
参考文献
  • Wakui H, et al. (1997) Interaction of the ligand-activated glucocorticoid receptor with the 14-3-3 eta protein. J Biol Chem. 272(13): 8153-6.
  • Toyooka K, et al. (2002) Isolation and structure of the mouse 14-3-3 eta chain gene and the distribution of 14-3-3 eta mRNA in the mouse brain. Brain Res Mol Brain Res. 100(1-2): 13-20.
  • Kim YS, et al. (2005) Role of 14-3-3 eta as a positive regulator of the glucocorticoid receptor transcriptional activation. Endocrinology. 146(7): 3133-40.
  • Grover D, et al. (2009) Family-based association of YWHAH in psychotic bipolar disorder. Am J Med Genet B Neuropsychiatr Genet. 150B(7): 977-83.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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