Anti-GST tag Antibody, Mouse Monoclonal

RRID Number: AB_2860323

Anti-GST tag Antibody, Mouse Monoclonal の製品情報

製品名
Anti-GST tag Antibody, Mouse Monoclonal
検証済のアプリケーション
WB,IP
交差反応
Reacts with: Schistosoma japonicum
特異性
Schistosoma japonicum GST tag
No cross-reactivity in ELISA with
E.coli cell lysate
免疫原
Recombinant GST protein
調製
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Glutathione S-transferase (GST). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
ソース
Monoclonal Mouse IgG1 Clone #6C10G4
精製
Protein A
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-GST tag Antibody, Mouse Monoclonal 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1/1000-1/10000
IP 1-4 μl/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-GST tag Antibody, Mouse Monoclonal の画像

Anti-GST Tag was immunoprecipitated using:

Lane A: GST-ARG1-Flag transfected 293 cell lysate (0.5mg)

Lane B: Flag-ARG1-GST transfected 293 cell lysate (0.5mg)

 

2 μg anti-HA Tag mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-GST Tag rabbit monoclonal antibody, at 1:100 dilution

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Recognize GST tag fusion protein human ERK2.

Anti-GST Tag mouse monoclonal antibody at 1:4000

Lane A: GST-ERK2 (Recombinant protein, Cat#10030-H09B) (100ng)

Lane B: GST-ERK2 (Recombinant protein, Cat#10030-H09B) (50ng)

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Recognize GST tag fusion protein human USP7.

Anti-GST Tag mouse monoclonal antibody at 1:4000

Lane A: GST-USP7 (Recombinant protein, Cat#11681-H20B) (100ng)

Lane B: GST-USP7 (Recombinant protein, Cat#11681-H20B) (50ng)

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Recognize GST tag fusion proteins which expressed by 293 cell.

Anti-GST Tag mouse monoclonal antibody at 1:1250 or 1:2500

Lane A: GST-FABP4-flag transfected 293 Cell Lysate (30ug)

Lane B: flag-FABP4-GST transfected 293 Cell Lysate (30ug)

Lane C: flag-AGR1-GST transfected 293 Cell Lysate (30ug)

Lane D: Negative control

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Recognize GST tag fusion proteins which expressed by E. coli.

Anti-GST Tag mouse monoclonal antibody at 1:25000, 1:1250

Lane A: GST-mBID transfected E.coli cell lysate (0.5ug)

Lane B: GST-mBID transfected E.coli cell lysate (0.2ug)

Lane C: GST-mBID transfected E.coli cell lysate (0.1ug)

Lane D: GST-mBID transfected E.coli cell lysate (0.05ug)

Lane E: GST-mBID transfected E.coli cell lysate (0.02ug)

Lane F: GST-YWHAB transfected E.coli cell lysate (0.5ug)

Lane G: GST-YWHAB transfected E.coli cell lysate (0.2ug)

Lane H: GST-YWHAB transfected E.coli cell lysate (0.1ug)

Lane I: GST-YWHAB transfected E.coli cell lysate (0.05ug)

Lane J: GST-YWHAB transfected E.coli cell lysate (0.02ug)

Lane K: Empty vecter (0.5ug)

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Anti-GST Tag mouse monoclonal antibody at 1:25000, 1:1250, 1:1000

Lane A: GST-mBID transfected E.coli cell lysate (0.1ug)

Lane B: GST-YWHAB transfected E.coli cell lysate (0.1ug)

Lane C: Empty vecter (0.1ug)

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Anti-GST Tag mouse monoclonal antibody at 1:25000, 1:1250, 1:25000

Lane A: GST-mBID transfected E.coli cell lysate (0.2ug)

Lane B: GST-YWHAB transfected E.coli cell lysate (0.2ug)

Lane C: Empty vecter (0.2ug)

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

GST tag 背景情報

The glutathione S-transferase (GST) tag is another well-established affinity tag based on the strong affinity of GST for immobilized glutathione. The GST tag is best suitable for use in prokaryotic expression because GSTs are a family of multifunctional cytosolic proteins that are present in eukaryotic organisms but generally not found in bacteria. Similar to the MBP tag, GST tag has long been used to increase the solubility of fusion proteins in E. coli. GST-tagged proteins are captured by immobilized glutathione and then are eluted under mild, nondenaturing conditions using reduced glutathione.
参考文献
  • Zhao X, et al. (2013) Several affinity tags commonly used in chromatographic purification. J Anal Methods Chem 2013 581093.
  • Monti M, et al. (2007) Functional proteomics: Protein-protein interactions in vivo. Ital J Biochem 56 (4): 310-314.
  • Brothers SP, et al. (2003) Unexpected effects of epitope and chimeric tags on gonadotropin-releasing hormone receptors: Implications for understanding the molecular etiology of hypogonadotropic hypogonadism. J Clin Endocrinol Metab 88 (12): 6107-6112.
  • 微小隐孢子虫 Ⅰ 型跨膜蛋白 Cgd7_4560 的亚细胞定位及其对 HCT-8 细胞的黏附特性
    Author
    邵清延, ;王科杰, ;王东强, ;张天宇, ;焦新, ;
    Year
    2019
    Journal
    吉林农业大学
  • Neddylation-mediated Nedd4-2 activation regulates ubiquitination modification of renal NBCe1
    Author
    Tu, J;Zhang, B;Fang, G;Chang, W;Zhao, Y;
    Year
    2020
    Journal
    Exp. Cell Res.

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