|Microneutralizaiton (MN)：||The neutralization activity is Measured by microneutralization assay in vitro. The virus microneutralizaiton (MN) test was performed on 293T-ACE2 cells infected with SARS-CoV-2 (2019-nCoV) Spike Pseudovirus under treatment of serial dilutions of neutralizing antibody. The infection was neutralized by increasing concentrations of Anti-SARS-CoV-2 Neutralizing Antibody (Catalog# 40592-R001). Rate of inhibition was determined by comparing the Relative Light Unit (RLU) of Luciferase reporter in different antibody concentrations. The IC50 is typically 0.11 µg/mL.|
Anti-SARS-CoV-2 Spike rabbit monoclonal antibody at 1:1000 dilution.
Lane A: SARS-CoV Spike/RBD Protein (40150-V08B2)(30ng)
Lane B: SARS-CoV-2 (2019-nCoV) Spike RBD-His Recombinant Protein (40592-V08B)(30ng)
Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.
Developed using the ECL technique.
Performed under reducing conditions.