SARS-CoV-2 (2019-nCoV) Spike S1 Antibody, Rabbit MAb

RRID Number: AB_2827979
COVID-19 Spike Research.
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SARS-CoV-2 (2019-nCoV) Spike S1 Antibody, Rabbit MAb の製品情報

製品名
SARS-CoV-2 (2019-nCoV) Spike S1 Antibody, Rabbit MAb
検証済のアプリケーション
ELISA
アプリケーションの説明
IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.)
特異性
2019-nCoV Coronavirus spike
Has cross-reactivity in ELISA with
SARS-CoV Spike S1 Protein (Cat# 40150-V08B1)
SARS-CoV Spike RBD Protein (Cat# 40150-V08B2)
No cross-reactivity in ELISA with
MERS-CoV Spike S1 Protein (Cat# 40069-V08B1)
HCoV-HKU1 (isolate N1) Spike S1 Protein (Cat# 40021-V08H)
HCoV-HKU1 (isolate N5) Spike S1 Protein (Cat# 40602-V08H)
HCoV-NL63 Spike S1 Protein (Cat# 40600-V08H)
HCoV-229E Spike S1 Protein (Cat# 40601-V08H)
HCoV-OC43 Spike S1+S2 ECD Protein (Cat# 40607-V08B)
調製
This product is a recombinant monoclonal antibody expressed from HEK293 cells.
ソース
Monoclonal Rabbit IgG Clone #007
精製
Protein A
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

SARS-CoV-2 (2019-nCoV) Spike S1 Antibody, Rabbit MAb 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
ELISA 1:5000-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

SARS-CoV-2 (2019-nCoV) Spike S1 Antibody, Rabbit MAb の画像

Immunofluorescence staining of SARS-CoV-2 (2019-nCoV) Spike in ACE2-overexpressed 293T cells, infected (left) or noninfected (right) by 2019-nCOV-Spike pseudovirus (PSV). Cells were blocked with 10% serum, and incubated with SARS-CoV-2 (2019-nCoV) Spike rabbit monoclonal antibody (dilution ratio 1:60) at 37℃ for 1 hour. Then cells were stained with the Alexa Fluor®-conjugated Goat Anti-rabbit IgG secondary antibody (green).

SARS-CoV-2 (2019-nCoV) Spike S1 Antibody, Rabbit MAb: 別名

Anti-coronavirus spike Antibody; Anti-cov spike Antibody; Anti-ncov RBD Antibody; Anti-ncov s1 Antibody; Anti-ncov s2 Antibody; Anti-ncov spike Antibody; Anti-NCP-CoV RBD Antibody; Anti-NCP-CoV s1 Antibody; Anti-NCP-CoV s2 Antibody; Anti-NCP-CoV Spike Antibody; Anti-novel coronavirus RBD Antibody; Anti-novel coronavirus s1 Antibody; Anti-novel coronavirus s2 Antibody; Anti-novel coronavirus spike Antibody; Anti-RBD Antibody; Anti-S1 Antibody; Anti-S2 Antibody; Anti-Spike RBD Antibody

Coronavirus spike 背景情報

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
参考文献
  • Shen S, et al. (2007) Expression, glycosylation, and modification of the spike (S) glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35.
  • Du L, et al. (2009) The spike protein of SARS-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36.
  • Xiao X, et al. (2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30.
  • Lectin-like Intestinal Defensin Inhibits 2019-nCoV Spike binding to ACE2
    Author
    Wang, C;Wang, S;Li, D;Zhao, X;Han, S;Wang, T;Zhao, G;
    Year
    2020
    Journal
    bioRxiv
  • Rapid Detection of COVID-19 Causative Virus (SARS-CoV-2) in Human Nasopharyngeal Swab Specimens Using Field-Effect Transistor-Based Biosensor
    Author
    Seo, G;Lee, G;Kim, MJ;Baek, SH;Choi, M;Ku, KB;Lee, CS;Jun, S;Park, D;Kim, HG;Kim, SJ;Lee, JO;Kim, BT;Park, EC;Kim, SI;
    Year
    2020
    Journal
    ACS Nano
  • SARS-CoV-2 invades host cells via a novel route: CD147-spike protein
    Author
    Wang, K;Chen, W;Zhou, YS;Lian, JQ;Zhang, Z;Du, P;
    Year
    2020
    Journal
    bioRxiv
    Application
    IP, ELISA, EM
  • 上海 2 株严重急性呼吸综合征冠状病毒 2 的分离与鉴定
    Author
    彭浩然, ;李成忠, ;唐海琳, ;薛建亚, ;陈志辉, ;梁雪松, ;朱咏梅, ;夏炳辉, ;朱勇喆, ;吴俊杰, ;赵兰娟, ;任浩, ;江亮亮, ;何燕华, ;戚中田, ;赵平, ;
    Year
    2020
    Journal
    第二军医大学学报
    Application
    IF
  • SARS-CoV-2 growth, furin-cleavage-site adaptation and neutralization using serum from acutely infected, hospitalized COVID-19 patients
    Author
    Klimstra, W;Tilston-Lunel, N;Nambulli, S;Boslett, J;McMillen, C;Gilliland, T;Dunn, M;Sun, C;Wheeler, S;Wells, A;Hartman, A;McElroy, A;Reed, D;Rennick, L;Duprex, W;
    Year
    2020
    Journal
    bioRxiv
    Application
    IF
  • Clinical and pathological findings of SARS-CoV-2 infection and concurrent IgA nephropathy: A case report
    Author
    Liu, L;
    Year
    2020
    Journal
    Research Square
    Application
    IHC
  • Unlocking the lockdown of science and demystifying COVID-19: how autopsies contribute to our understanding of a deadly pandemic
    Author
    Tzankov, A;Jonigk, D;
    Year
    2020
    Journal
    Virchows Arch.
    Application
    IHC

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