Human coronavirus (HCoV-NL63) Antibody, Rabbit PAb, Antigen Affinity Purified

Human coronavirus (HCoV-NL63) Antibody, Rabbit PAb, Antigen Affinity Purified の製品情報

製品名
Human coronavirus (HCoV-NL63) Antibody, Rabbit PAb, Antigen Affinity Purified
検証済のアプリケーション
WB,ELISA
アプリケーションの説明
IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.)
特異性
HCoV-NL63 Coronavirus Nucleocapsid
免疫原
Recombinant Human coronavirus (HCoV-NL63) Nucleoprotein / NP Protein (Catalog#40641-V07E)
調製
Produced in rabbits immunized with purified, recombinant Human coronavirus (HCoV-NL63) Nucleoprotein / NP ( Catalog#40641-V07E; YP_003771.1; Met1-His377). Coronavirus Nucleocapsid specific IgG was purified by Human coronavirus (HCoV-NL63) Nucleoprotein / NP affinity chromatography.
ソース
Polyclonal Rabbit IgG
精製
Protein A & Antigen Affinity
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Human coronavirus (HCoV-NL63) Antibody, Rabbit PAb, Antigen Affinity Purified 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:2000-1:10000
ELISA 1:5000-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Human coronavirus (HCoV-NL63) Antibody, Rabbit PAb, Antigen Affinity Purified の画像

Anti-HCoV NL63 NP rabbit polyclonal antibody at 1:2000 dilution.

Sample:Human coronavirus (HCoV-NL63) Nucleoprotein / NP Protein

Lane A: 5ng

Lane B: 1ng

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Coronavirus Nucleocapsid 背景情報

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
参考文献
    1. 1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12.
    2. Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6.
    3. Li W. et al., 2005, Science. 309 (5742): 1864-8.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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