SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody, Rabbit MAb

RRID Number: AB_2857931
EliteRmab®, manufactured by recombinant technology

SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody, Rabbit MAb の製品情報

製品名
SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody, Rabbit MAb
検証済のアプリケーション
WB,ELISA
アプリケーションの説明
(Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
特異性
SARS-CoV-2 Coronavirus Nucleocapsid
Has cross-reactivity in ELISA and WB with
MERS-CoV Nucleocapsid Protein (His Tag) (Cat# 40068-V08B)

Has cross-reactivity in ELISA with
SARS-CoV Nucleocapsid Protein (His Tag) (Cat# 40143-V08B)
No cross-reactivity in ELISA with
HCoV-229E Nucleoprotein protein (40640-V07E)
HCoV-NL63 Nucleoprotein protein (40641-V07E)
HCoV-HKU1(isolate N5) Nucleoprotein protein (40642-V07E)
HCoV-OC43 Nucleoprotein (40643-V07E)
Influenza A H1N1 (A/California/07/2009) Nucleocapsid Protein (His Tag) (Catalog#40205-V08B)
Influenza A H3N2 (A/Switzerland/9715293/2013) Nucleocapsid Protein (His Tag) (Catalog#40499-V08B)
Influenza A H2N2 Nucleocapsid Protein (His Tag) (Catalog#40033-V08B)
Influenza A H7N9 (A/Shanghai/2/2013) Nucleocapsid Protein (His Tag) (Catalog#40111-V08B)
Influenza B (B/Florida/4/2006) Nucleocapsid Protein (His Tag) (Catalog#40438-V08B)
免疫原
Recombinant SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (Catalog#40588-V08B)
調製
This product is a recombinant monoclonal antibody expressed from HEK293 cells.
ソース
Monoclonal Rabbit IgG Clone #0004
精製
Protein A
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody, Rabbit MAb 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:500-1:2000
ELISA 1:5000-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody, Rabbit MAb の画像

Anti-SARS-NP rabbit monoclonal antibody at 1:1000 dilution.

Lane A: SARS-CoV-2 (2019-nCoV) Nucleocapsid (40588-V08B) (30ng)

Lane B: MERS-CoV Nucleocapsid (40068-V08B) (30ng)

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Coronavirus Nucleocapsid 背景情報

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
参考文献
    1. 1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12.
    2. Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6.
    3. Li W. et al., 2005, Science. 309 (5742): 1864-8.

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