SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody, Mouse MAb

SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody, Mouse MAb の製品情報

製品名
SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody, Mouse MAb
検証済のアプリケーション
ELISA,ELISA(Cap)
アプリケーションの説明
IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.)
特異性
SARS-CoV-2 Coronavirus Nucleocapsid
免疫原
Recombinant SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (Catalog#40588-V08B)
調製
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, SARS-CoV-2 (2019-nCoV) Nucleocapsid (Catalog#40588-V08B; YP_009724397.2(335Gly/Ala); Met1-Ala419). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
ソース
Monoclonal Mouse IgG1 Clone #122
精製
Protein A
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody, Mouse MAb 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
ELISA 1:1000-1:2000
ELISA(Cap) 1:250-1:2000
Notes
ELISA(Cap): In a sandwich ELISA, Cat# 40588-MM122 can be used as capture antibody when paired with Cat# 40588-MM128.
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Coronavirus Nucleocapsid 背景情報

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
参考文献
    1. 1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12.
    2. Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6.
    3. Li W. et al., 2005, Science. 309 (5742): 1864-8.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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