SARS-CoV / SARS-CoV-2 Nucleoprotein / NP Antibody, Rabbit PAb, Antigen Affinity Purified

SARS-CoV / SARS-CoV-2 Nucleoprotein / NP Antibody, Rabbit PAb, Antigen Affinity Purified の製品情報

製品名
SARS-CoV / SARS-CoV-2 Nucleoprotein / NP Antibody, Rabbit PAb, Antigen Affinity Purified
検証済のアプリケーション
WB,ELISA
アプリケーションの説明
IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.)
特異性
SARS Coronavirus Nucleocapsid
Has cross-reactivity in ELISA and WB with
SARS-CoV-2 (2019-nCoV) Nucleoprotein / NP Protein (Cat# 40588-V08B).
免疫原
Recombinant SARS-CoV Nucleoprotein / NP Protein (Catalog#40143-V08B)
調製
Produced in rabbits immunized with purified, recombinant SARS-CoV Nucleoprotein / NP (Catalog#40143-V08B; NP_828858.1; Met1-Ala422). SARS-CoV Nucleoprotein / NP specific IgG was purified by SARS-CoV Nucleoprotein / NP affinity chromatography.
ソース
Polyclonal Rabbit IgG
精製
Protein A & Antigen Affinity
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

SARS-CoV / SARS-CoV-2 Nucleoprotein / NP Antibody, Rabbit PAb, Antigen Affinity Purified 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:1000-1:5000
ELISA 1:5000-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

SARS-CoV / SARS-CoV-2 Nucleoprotein / NP Antibody, Rabbit PAb, Antigen Affinity Purified の画像

Anti-SARS-NP rabbit polyclonal antibody at 1:2000 dilution.

Lane A: SARS-CoV NP Protein (Cat#40143-V08B) (30ng)

Lane B: SARS-CoV NP Protein (Cat#40143-V08B) (5ng)

Lane C: SARS-CoV-2 (2019-nCoV) NP Protein (Cat#40588-V08B) (30ng)

Lane D: SARS-CoV-2 (2019-nCoV) NP Protein (Cat#40588-V08B) (5ng)

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

SARS-CoV / SARS-CoV-2 Nucleoprotein / NP Antibody, Rabbit PAb, Antigen Affinity Purified: 別名

Anti-coronavirus NP Antibody; Anti-coronavirus Nucleocapsid Antibody; Anti-coronavirus Nucleoprotein Antibody; Anti-cov np Antibody; Anti-ncov NP Antibody; Anti-novel coronavirus NP Antibody; Anti-novel coronavirus Nucleocapsid Antibody; Anti-novel coronavirus Nucleoprotein Antibody; Anti-NP Antibody; Anti-Nucleocapsid Antibody; Anti-Nucleoprotein Antibody

Coronavirus Nucleocapsid 背景情報

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
参考文献
    1. 1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12.
    2. Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6.
    3. Li W. et al., 2005, Science. 309 (5742): 1864-8.
  • Comparative pathogenesis of COVID-19, MERS, and SARS in a nonhuman primate model
    Author
    Rockx, B;Kuiken, T;Herfst, S;Bestebroer, T;Lamers, MM;Oude Munnink, BB;de Meulder, D;van Amerongen, G;van den Brand, J;Okba, NMA;Schipper, D;van Run, P;Leijten, L;Sikkema, R;Verschoor, E;Verstrepen, B;Bogers, W;Langermans, J;Drosten, C;Fentener van Vlissingen, M;Fouchier, R;de Swart, R;Koopmans, M;Haagmans, BL;
    Year
    2020
    Journal
    Science
    Application
    IHC
  • Evidence for gastrointestinal infection of SARS-CoV-2
    Author
    Xiao, F;Tang, M;Zheng, X;Liu, Y;Li, X;Shan, H;
    Year
    2020
    Journal
    Gastroenterology
  • Renal histopathological analysis of 26 postmortem findings of patients with COVID-19 in China
    Author
    Su, H;Yang, M;Wan, C;Yi, LX;Tang, F;Zhu, HY;Yi, F;Yang, HC;Fogo, AB;Nie, X;Zhang, C;
    Year
    2020
    Journal
    Kidney Int.
    Application
    IF
  • Generation of a Broadly Useful Model for COVID-19 Pathogenesis, Vaccination, and Treatment
    Author
    Sun, J;Zhuang, Z;Zheng, J;Li, K;Wong, R;Liu, D;Huang, J;He, J;Zhu, A;Zhao, J;Li, X;Xi, Y;Chen, R;Alshukairi, A;Chen, Z;Zhang, Z;Chen, C;Huang, X;Li, F;Lai, X;Chen, D;Wen, L;Zhuo, J;Zhang, Y;Wang, Y;Huang, S;Dai, J;Shi, Y;Zheng, K;Leidinger, M;Chen, J;Li, Y;Zhong, N;Meyerholz, D;McCray, P;Perlman, S;Zhao, J;
    Year
    2020
    Journal
    Cell
    Application
    FFA
  • Neuropathology of patients with COVID-19 in Germany: a post-mortem case series
    Author
    Matschke, J;Lütgehetmann, M;Hagel, C;Sperhake, JP;Schröder, AS;Edler, C;Mushumba, H;Fitzek, A;Allweiss, L;Dandri, M;Dottermusch, M;Heinemann, A;Pfefferle, S;Schwabenland, M;Sumner Magruder, D;Bonn, S;Prinz, M;Gerloff, C;Püschel, K;Krasemann, S;Aepfelbacher, M;Glatzel, M;
    Year
    2020
    Journal
    Lancet Neurol

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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