SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody, Mouse MAb

RRID Number: AB_2827977
COVID-19 Nucleocapsid Research.
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SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody, Mouse MAb の製品情報

製品名
SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody, Mouse MAb
検証済のアプリケーション
WB,ELISA
アプリケーションの説明
IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.)
特異性
SARS Coronavirus Nucleocapsid
Has cross-reactivity in ELISA and WB with
SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (Cat# 40588-V08B)
No cross-reactivity in ELISA with
MERS-CoV Nucleoprotein protein (40068-V08B)
HCoV-229E Nucleoprotein protein (40640-V07E)
HCoV-NL63 Nucleoprotein protein (40641-V07E)
HCoV-HKU1(isolate N5) Nucleoprotein protein (40642-V07E)
HCoV-OC43 Nucleoprotein (40643-V07E)
免疫原
Recombinant SARS-CoV Nucleoprotein / NP Protein (Catalog#40143-V08B)
調製
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant SARS-CoV Nucleoprotein / NP (Catalog#40143-V08B; NP_828858.1; Met1-Ala422). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
ソース
Monoclonal Mouse IgG1 Clone #05
精製
Protein A
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody, Mouse MAb 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:1000-1:5000
ELISA 1:1000-1:2000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody, Mouse MAb の画像

Anti-SARS-NP mouse monoclonal antibody at 1:1000 dilution.

Lane A: SARS-CoV NP Protein (Cat#40143-V08B) (30ng)

Lane B: SARS-CoV NP Protein (Cat#40143-V08B) (5ng)

Lane C: SARS-CoV-2 (2019-nCoV) NP Protein (Cat#40588-V08B) (30ng)

Lane D: SARS-CoV-2 (2019-nCoV) NP Protein (Cat#40588-V08B) (5ng)

Secondary

Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody, Mouse MAb: 別名

Anti-coronavirus NP Antibody; Anti-coronavirus Nucleocapsid Antibody; Anti-coronavirus Nucleoprotein Antibody; Anti-cov np Antibody; Anti-ncov NP Antibody; Anti-novel coronavirus NP Antibody; Anti-novel coronavirus Nucleocapsid Antibody; Anti-novel coronavirus Nucleoprotein Antibody; Anti-NP Antibody; Anti-Nucleocapsid Antibody; Anti-Nucleoprotein Antibody

Coronavirus Nucleocapsid 背景情報

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
参考文献
    1. 1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12.
    2. Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6.
    3. Li W. et al., 2005, Science. 309 (5742): 1864-8.
  • Molecular Detection of SARS-CoV-2 in Formalin Fixed Paraffin Embedded Specimens
    Author
    Zeng, X;Liu, J;Babka, A;Kearney, B;Radoshitzky, S;
    Year
    2020
    Journal
    bioRxiv
  • Critical Role of Type III Interferon in Controlling SARS-CoV-2 Infection in Human Intestinal Epithelial Cells
    Author
    Stanifer, ML;Kee, C;Cortese, M;Zumaran, CM;Triana, S;Mukenhirn, M;Kraeusslich, HG;Alexandrov, T;Bartenschlager, R;Boulant, S;
    Year
    2020
    Journal
    Cell Rep
    Application
    IF; WB;
  • SARS-CoV-2 infection and replication in human fetal and pediatric gastric organoids
    Author
    Giobbe, G;Bonfante, F;Zambaiti, E;Gagliano, O;Jones, B;Luni, C;Laterza, C;Perin, S;Stuart, H;Pagliari, M;Bortolami, A;Mazzetto, E;Manfredi, A;Colantuono, C;Di Filippo, L;Pellegata, A;Li, V;Eaton, S;Thapar, N;Cacchiarelli, D;Elvassore, N;De Coppi, P;
    Year
    2020
    Journal
    bioRxiv
    Application
    Elisa
  • SARS-CoV-2 induces activation and diversification of human plasmacytoid pre-dendritic cells
    Author
    Onodi, F;Bonnet-Madin, L;Karpf, L;Meertens, L;Poirot, J;Legoff, J;Delaugerre, C;Amara, A;Soumelis, V;
    Year
    2020
    Journal
    bioRxiv
    Application
    FCM
  • Human angiotensin-converting enzyme 2 transgenic mice infected with SARS-CoV-2 develop severe and fatal respiratory disease
    Author
    Golden, J;Cline, C;Zeng, X;Garrison, A;Carey, B;Mucker, E;White, L;Shamblin, J;Brocato, R;Liu, J;Babka, A;Rauch, H;Smith, J;Hollidge, B;Fitzpatrick, C;Badger, C;Hooper, J;
    Year
    2020
    Journal
    bioRxiv
  • Structures, conformations and distributions of SARS-CoV-2 spike protein trimers on intact virions
    Author
    Ke, Z;Oton, J;Qu, K;Cortese, M;Zila, V;McKeane, L;Nakane, T;Zivanov, J;Neufeldt, C;Lu, J;Peukes, J;Xiong, X;Kräusslich, H;Scheres, S;Bartenschlager, R;Briggs, J;
    Year
    2020
    Journal
    bioRxiv
    Application
    WB

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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