MERS-CoV Nucleocapsid Protein Antibody, Rabbit PAb, Antigen Affinity Purified

MERS-CoV Nucleocapsid Protein Antibody, Rabbit PAb, Antigen Affinity Purified の製品情報

製品名
MERS-CoV Nucleocapsid Protein Antibody, Rabbit PAb, Antigen Affinity Purified
検証済のアプリケーション
WB
アプリケーションの説明
IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.)
特異性
MERS-CoV Coronavirus Nucleocapsid
免疫原
A synthetic peptide corresponding to the center region of MERS-CoV (NCoV / Novel coronavirus) Nucleocapsid Protein (NP protein).
調製
Produced in rabbits immunized with purified, a synthetic peptide corresponding to the center region of MERS-CoV (NCoV / Novel coronavirus) Nucleocapsid Protein (NP protein), and purified by antigen affinity chromatography.
ソース
Polyclonal Rabbit IgG
精製
Protein A & Antigen Affinity
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

MERS-CoV Nucleocapsid Protein Antibody, Rabbit PAb, Antigen Affinity Purified 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:1000-1:5000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

MERS-CoV Nucleocapsid Protein Antibody, Rabbit PAb, Antigen Affinity Purified の画像

Anti-Novel coronavirus (HCoV-EMC/2012) Nucleoprotein / NP rabbit polyclonal antibody at 1:1000 dilition.

Sample:Novel coronavirus (HCoV-EMC/2012) Nucleoprotein / NP

Lane A: 500ng

Lane B: 200ng

Lane C: 100ng

Secondary

Goat Anti- Rabbit IgG (H+L)/HRP at 0.4ug/ml dilution.

Performed under reducing conditions.

MERS-CoV Nucleocapsid Protein Antibody, Rabbit PAb, Antigen Affinity Purified: 別名

Anti-coronavirus NP Antibody; Anti-coronavirus Nucleocapsid Antibody; Anti-coronavirus Nucleoprotein Antibody; Anti-cov np Antibody; Anti-ncov NP Antibody; Anti-novel coronavirus Nucleoprotein Antibody; Anti-NP Antibody; Anti-Nucleocapsid Antibody; Anti-Nucleoprotein Antibody

Coronavirus Nucleocapsid 背景情報

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
参考文献
    1. 1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12.
    2. Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6.
    3. Li W. et al., 2005, Science. 309 (5742): 1864-8.
  • Recombinant Receptor Binding Domain Protein Induces Partial Protective Immunity in Rhesus Macaques Against Middle East Respiratory Syndrome Coronavirus Challenge
    Author
    Lan, J;Yao, Y;Deng, Y;Chen, H;Lu, G;Wang, W;Bao, L;
    Year
    2015
    Journal
    EBioMedicine
    Application
    IHC
  • Recombinant Receptor Binding Domain Protein Induces Partial Protective Immunity in Rhesus Macaques Against Middle East Respiratory Syndrome Coronavirus Challenge
    Author
    Lan, J;Yao, Y;Deng, Y;Chen, H;Lu, G;Wang, W;Bao, L;
    Year
    2015
    Journal
    EBioMedicine
    Application
    IHC
  • The recombinant N-terminal domain of spike proteins is a potential vaccine against Middle East respiratory syndrome coronavirus (MERS-CoV) infection
    Author
    Jiaming, L;Yanfeng, Y;Yao, D;Yawei, H;Linlin, B;Baoying, H;Jinghua, Y;Gao, GF;Chuan, Q;Wenjie, T;
    Year
    2017
    Journal
    Vaccine
    Application
    IHC
  • Enhanced protection in mice induced by immunization with inactivated whole viruses compare to spike protein of middle east respiratory syndrome coronavirus
    Author
    Deng, Y;Lan, J;Bao, L;Huang, B;Ye, F;Chen, Y;Yao, Y;Wang, W;Qin, C;Tan, W;
    Year
    2018
    Journal
    Emerg Microbes Infect
    Application
    IHC

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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