Beta-Tubulin Loading Control Antibody, Mouse MAb

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Beta-Tubulin Loading Control Antibody, Mouse MAb (Mouse Monoclonal 抗体) の製品情報

製品名
Beta-Tubulin Loading Control Antibody, Mouse MAb
検証済のアプリケーション
WB,ICC/IF
交差反応
Reacts with: Human
特異性
Human Beta-Tubulin
免疫原
A synthetic peptide corresponding to the N-terminus of the human beta-tubulin
調製
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the N-terminus of the human beta-tubulin. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
ソース
Monoclonal Mouse IgG2b Clone #05
精製
Protein A
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Beta-Tubulin Loading Control Antibody, Mouse MAb (Mouse Monoclonal 抗体) 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1/1000-1/2000
ICC-IF 1/50-1/200
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Beta-Tubulin Loading Control Antibody, Mouse MAb (Mouse Monoclonal 抗体) の画像

Immunofluorescence staining of Human Beta-Tubulin in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human Beta-Tubulin monoclonal antibody at 4℃ overnight. Then cells were stained with the Alexa Fluor® 594-conjugated (left panel, captured by laser confocal scanning microscope) and Alexa Fluor®488-conjugated (right panel, captured by fluorescence microscope) Goat Anti-mouse IgG secondary antibody, countstained with DAPI (blue). Positive staining was localized to cytoskeleton.

Beta-Tubulin 背景情報

Beta-Tubulin is a subunit of tubulin. Tubulin is one of several members of a small family of globular proteins. It is the major constituent of microtubules. There are two most common members of the tubulin family: alpha-tubulin and beta-tubulin, and together their dimers form microtubules. The dimers of alpha- and beta-tubulin bind to GTP and assemble onto the (+) ends of microtubules while in the GTP-bound state. After the dimer is incorporated into the microtubule, the molecule of GTP bound to the beta -tubulin subunit eventually hydrolyzes into GDP through inter-dimer contacts along the microtubule protofilament. Beta-tubulin faces the plus end of the microtubule while alpha-tubulin faces the minus end. Dimers bound to GTP tend to assemble into microtubules, while dimers bound to GDP tend to fall apart. Loading controls are usually proteins that exhibit high-level, constitutive expression in the cell type or sample you are examining. This ensures constant expression levels. Thus “housekeeping genes” are frequently chosen for use as loading controls. It is also important that the protein chosen as a loading control has a different molecular weight than the protein of interest so that the bands are distinct and expression levels quantifiable. Popular loading control detection antibodies include anti-β-Actin monoclonal or polyclonal antibodies, anti-COX-4, anti-GAPDH, anti-Tubulin and anti-VDAC/Porin antibodies.
参考文献
  • Williams RC Jr. et al., 1999. Anal Biochem. 275(2): 265-7.
  • Nogales E. et al., 1998. Nat Struct Biol. 5(6): 451-8.
  • Dutcher SK. 2001. Curr Opin Cell Biol. 13(1): 49-54.
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    Author
    Li, G;Chen, H;Cheng, L;Zhao, R;Zhao, J;Xu, Y;
    Year
    2014
    Journal
    Neural Regen Res
    Application
    WB
  • Design, synthesis and biological evaluation of shikonin thio-glycoside derivatives: new anti-tubulin agents
    Author
    HY Lin, HW Han, LF Bai, HY Qiu, DZ Yin, JL Qi…
    Year
    2014
    Journal
    RSC Advances
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    Author
    Zhuang, M;Li, B;Huang, Y;Lei, Q;Yan, R;Li, N;Sidhu, K;Cheng, X;Yan, X;Miao, Y;Zhao, S;Hua, J;
    Year
    2018
    Journal
    J. Cell. Biochem.
    Application
    WB

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