Anti-EGFR Antibody

1/1
Price:
Size:
Number:

Anti-EGFR Antibody (Rabbit Polyclonal 抗体) の製品情報

製品名
Anti-EGFR Antibody
検証済のアプリケーション
WB,ELISA
交差反応
Reacts with: Human
特異性
Human EGFR
免疫原
Recombinant Human EGFR Protein (Catalog#10001-H08B)
調製
Produced in rabbits immunized with purified, recombinant Human EGFR (rh EGFR; Catalog#10001-H08B; NP_005219; Met1-Lys642). EGFR specific IgG was purified by Human EGFR affinity chromatography.
ソース
Polyclonal Rabbit IgG
精製
Protein A & Antigen Affinity
緩衝液
PBS, pH7.0 with 0.03% Proclin300
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.

Anti-EGFR Antibody (Rabbit Polyclonal 抗体) 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:500-1:2000
ELISA 1:5000-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-EGFR Antibody (Rabbit Polyclonal 抗体) の画像

Anti-EGFR rabbit polyclonal antibody at 1:500 dilution

Lane A: HeLa Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:134 kDa

Anti-EGFR Antibody: 別名

Anti-ERBB Antibody; Anti-ERBB1 Antibody; Anti-HER1 Antibody; Anti-mENA Antibody; Anti-NISBD2 Antibody; Anti-PIG61 Antibody

EGFR 背景情報

As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.
完全な名称
epidermal growth factor receptor
参考文献
  • Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell 103(2): 211-25.
  • Giaccone, G. (2005) HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann. Oncol. 16(4): 538-48.
  • Yarden, Y., et al. (2001) Untangling the ErbB signalling network. Nat. Rev. Mol. Cell. Biol. 2(2): 127-37.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

カートに追加しました カートに追加しませんでした カートを更新しています。少々お待ちください