Anti-C-Reactive Protein Antibody

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Anti-C-Reactive Protein Antibody (Rabbit Polyclonal 抗体) の製品情報

製品名
Anti-C-Reactive Protein Antibody
検証済のアプリケーション
WB,ELISA,ICC/IF,IP
交差反応
Reacts with: Human
特異性
Human C-Reactive Protein
免疫原
Recombinant Human C-Reactive Protein/CRP Protein (Catalog#11250-HNAH)
調製
Produced in rabbits immunized with purified, recombinant Human C-Reactive Protein/CRP (rh C-Reactive Protein/CRP; Catalog#11250-HNAH; P02741-1; Met1-Pro224). C-Reactive Protein/CRP specific IgG was purified by Human C-Reactive Protein/CRP affinity chromatography.
ソース
Polyclonal Rabbit IgG
精製
Protein A & Antigen Affinity
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-C-Reactive Protein Antibody (Rabbit Polyclonal 抗体) 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:500-1:2000
ELISA 1:5000-1:10000
ICC-IF 1:100-1:500
IP IP:0.5-2μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-C-Reactive Protein Antibody (Rabbit Polyclonal 抗体) の画像

Anti-CRP rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lane B: SH-SY5Y Whole Cell Lysate

Lane C: Jurkat Whole Cell Lysate

Lane D: HeLa Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:25 kDa

Observed band size:27 kDa

Immunofluorescence staining of CRP in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human CRP polyclonal antibody (dilution ratio 1:200) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.

CRP was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

4 µL anti-CRP rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-CRP rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 25 kDa

Observed band size :26 kDa

Anti-C-Reactive Protein Antibody: 別名

Anti-C-Reactive Protein Antibody; Anti-PTX1 Antibody

C-Reactive Protein 背景情報

C-reactive protein (CRP) is synthesized by the liver in response to factors released by fat cells. It is a member of the pentraxin family of proteins. The levels of CRP rise in response to inflammation. Human C-reactive protein (CRP) is the classical acute phase reactant, the circulating concentration of which rises rapidly and extensively in a cytokine-mediated response to tissue injury, infection and inflammation. Serum CRP values are routinely measured, empirically, to detect and monitor many human diseases. However, CRP is likely to have important host defence, scavenging and metabolic functions through its capacity for calcium-dependent binding to exogenous and autologous molecules containing phosphocholine (PC) and then activating the classical complement pathway. CRP may also have pathogenic effects and the recent discovery of a prognostic association between increased CRP production and coronary atherothrombotic events is of particular interest.
完全な名称
C-reactive protein, pentraxin-related
参考文献
  • Pepys MB. et al., 2003, J Clin Invest. 111 (12): 1805-12.
  • Thompson D. et al., 1999, Structure. 7(2): 169-77.

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