Anti-ACVR2A Antibody

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Anti-ACVR2A Antibody (Rabbit Polyclonal 抗体) の製品情報

製品名
Anti-ACVR2A Antibody
検証済のアプリケーション
WB,ELISA,IP
交差反応
Reacts with: Human
特異性
Human ACVR2A
免疫原
Recombinant Human ACVR2A Protein (Catalog#10257-H08H)
調製
Produced in rabbits immunized with purified, recombinant Human ACVR2A (rh ACVR2A; Catalog#10257-H08H; NP_001607.1; Met1-Pro134). ACVR2A specific IgG was purified by Human ACVR2A affinity chromatography.
ソース
Polyclonal Rabbit IgG
精製
Protein A & Antigen Affinity
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-ACVR2A Antibody (Rabbit Polyclonal 抗体) 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:500-1:2000
ELISA 1:5000-1:10000
IP 1-5 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-ACVR2A Antibody (Rabbit Polyclonal 抗体) の画像

Anti-ACVR2A rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lane B: HeLa Whole Cell Lysate

Lane C: K562 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:58 kDa

Observed band size:60 kDa

ACVR2A was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

Lane B:0.5 mg HeLa Whole Cell Lysate

Lane C:0.5 mg K562 Whole Cell Lysate

2 µL anti-ACVR2A rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-ACVR2A rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution

Developed using the technique.

Performed under reducing conditions.

Predicted band size: 58 kDa

Observed band size :58 kDa

Anti-ACVR2A Antibody: 別名

Anti-ACTRII Antibody; Anti-ACVR2 Antibody

ACVR2A 背景情報

ACVR2A and ACVR2B are two activin type II receptors. ACVR2A has been shown to interact with INHBA, SYNJ2BP and ACVR1B. The bovine ACVR2A gene encodes a protein of 513 amino acids which is highly homologous (approximately 98% identity) to the rat, mouse, and human ACVR2A proteins. Inactivation of ACVR2A is a common event in prostate cancer cells suggesting it may play an important role in the development of prostate cancer. The ACVR2A gene is a putative tumor suppressor gene that is frequently mutated in microsatellite-unstable colon cancers (MSI-H colon cancers). Frameshift mutation of ACVR2A may contribute to MSI-H colon tumorigenesis via disruption of alternate TGF-beta effector pathways.
完全な名称
activin A receptor, type IIA
参考文献
  • Albertson RC, et al. (2005) Zebrafish acvr2a and acvr2b exhibit distinct roles in craniofacial development. Developmental dynamics 233(4): 1405-18.
  • Chung H, et al. (2008) Mutation rates of TGFBR2 and ACVR2 coding microsatellites in human cells with defective DNA mismatch repair. PloS one 3(10): e3463.
  • Fitzpatrick E, et al. (2009) Genetic association of the activin A receptor gene (ACVR2A) and pre-eclampsia. Molecular human reproduction 15(3):195-204.
  • Roten LT, et al. (2009) Association between the candidate susceptibility gene ACVR2A on chromosome 2q22 and pre-eclampsia in a large Norwegian population-based study (the HUNT study). EJHG. 17(2): 250-7.
  • Updated protocols for the detection of Sotatercept and Luspatercept in human serum
    Author
    Reichel, C;Gmeiner, G;Walpurgis, K;Thevis, M;
    Year
    2018
    Journal
    Drug Test Anal
    Application
    IP

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