Granulin Protein, Human, Recombinant (His Tag)

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Granulin Protein, Human, Recombinant (His Tag): 製品情報

純度
> 95 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
活性
Testing in progress
タンパク質の構築
A DNA sequence encoding the human GRN (NP_002078.1) (Met 1-Leu 593) with a C-terminal polyhistidine tag was expressed.
NP No.
発現ホスト
HEK293 Cells
Human
予測N末端
Thr 18
分子量
The secreted recombinant human GRN comprises 587 amino acids with a predicted molecular mass of 63.2 kDa. As a result of glycosylation, rhGRN migrates as an approximately 100-105 kDa band in SDS-PAGE under reducing conditions.
バッファー
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Granulin Protein, Human, Recombinant (His Tag): 画像

Granulin Protein, Human, Recombinant (His Tag): 別名

CLN11 Protein, Human; GEP Protein, Human; GP88 Protein, Human; Granulin Protein, Human; PCDGF Protein, Human; PEPI Protein, Human; PGRN Protein, Human; Progranulin Protein, Human

Granulin 背景情報

Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis. Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling. Granulin-4 promotes proliferation of the epithelial cell line A431 in culture while granulin-3 acts as an antagonist to granulin-4, inhibiting the growth. Granulin expression inhibited Tat transactivation, and tethering experiments showed that this effect was due, at least in part, to a direct action on cyclin T1 in the absence of Tat.
完全な名称
Granulin Precursor
参考文献
  • Hoque M, et al. (2003) The growth factor granulin interacts with cyclin T1 and modulates P-TEFb-dependent transcription. Mol Cell Biol. 23(5): 1688-702.
  • Bateman A, et al. (1990) Granulins, a novel class of peptide from leukocytes. Biochem Biophys Res Commun. 173(3): 1161-8.
  • Trinh DP, et al. (1999) Epithelin/granulin growth factors: extracellular cofactors for HIV-1 and HIV-2 Tat proteins. Biochem Biophys Res Commun. 256(2): 299-306.
  • Assessing kinetic and epitopic diversity across orthogonal monoclonal antibody generation platforms
    Author
    Abdiche, YN;Harriman, R;Deng, X;Yeung, YA;Miles, A;Morishige, W;Boustany, L;Zhu, L;Izquierdo, SM;Harriman, W;
    Year
    2016
    Journal
    MAbs
    Application
    antigen
  • Tumor necrosis factor receptor 2/AKT and ERK signaling pathways contribute to the switch from fibroblasts to CAFs by progranulin in microenvironment of colorectal cancer
    Author
    Wang, L;Yang, D;Tian, J;Gao, A;Shen, Y;Ren, X;Li, X;Jiang, G;Dong, T;
    Year
    2017
    Journal
    Oncotarget
    Application
    cell-based (proliferation)
  • Progranulin Promotes Regeneration of Inflammatory Periodontal Bone Defect in Rats via Anti-inflammation, Osteoclastogenic Inhibition, and Osteogenic Promotion
    Author
    Chen, Q;Cai, J;Li, X;Song, A;Guo, H;Sun, Q;Yang, C;Yang, P;
    Year
    2018
    Journal
    Inflammation
    Application
    animal-based
  • CSF progranulin increases in the course of Alzheimer's disease and is associated with sTREM2, neurodegeneration and cognitive decline
    Author
    Suárez-Calvet, M;Capell, A;Araque Caballero, MÁ;Morenas-Rodríguez, E;Fellerer, K;Franzmeier, N;Kleinberger, G;Eren, E;Deming, Y;Piccio, L;Karch, CM;Cruchaga, C;Paumier, K;Bateman, RJ;Fagan, AM;Morris, JC;Levin, J;Danek, A;Jucker, M;Masters, CL;Rossor, MN;Ringman, JM;Shaw, LM;Trojanowski, JQ;Weiner, M;Ewers, M;Haass, C;, ;, ;
    Year
    2018
    Journal
    EMBO Mol Med
    Application
    ELISA (standard)
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