PD-1 Protein, Mouse, Recombinant (His Tag)

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PD-1 Protein, Mouse, Recombinant (His Tag): 製品情報

純度
> 95 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
活性
Testing in progress
タンパク質の構築
A DNA sequence encoding the extracellular domain (Met 1-Gln 167) of mouse PDCD1 (NP_032824.1) precursor was expressed with a polyhistidine tag at the C-terminus.
NP No.
発現ホスト
HEK293 Cells
Mouse
予測N末端
Leu 25
分子量
The secreted recombinant mouse PDCD1 consists of 154 amino acids and has a predicted molecular mass of 17.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm PDCD1 is approximately 40-45 kDa due to glycosylation.
バッファー
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

PD-1 Protein, Mouse, Recombinant (His Tag): 画像

PD-1 Protein, Mouse, Recombinant (His Tag): 別名

Ly101 Protein, Mouse; PD-1 Protein, Mouse; Pdc1 Protein, Mouse

PD-1 背景情報

Programmed cell death 1, also known as PDCD1, is a type I transmembrane glycoprotein, and is an immunoreceptor belonging to the CD28/CTLA-4 family negatively regulates antigen receptor signaling by recruiting protein tyrosine phosphatase, SHP-2 upon interacting with either of two ligands, PD-L1 or PD-L2. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-1 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance, and thus contributes to the prevention of autoimmune diseases. Furthermore, PD1 is shown to be a regulator of virus-specific CD8+ T cell survival in HIV infection. As a cell surface molecule, PDCD1 regulates the adaptive immune response. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function.
完全な名称
programmed cell death 1
参考文献
  • James ES, et al. (2005) PDCD1: a tissue-specific susceptibility locus for inherited inflammatory disorders. Genes Immun. 6(5): 430-7.
  • Okazaki T, et al. (2007) PD-1 and PD-1 ligands: from discovery to clinical application. Int Immunol. 19(7): 813-24.
  • del Rio ML, et al. (2008) PD-1/PD-L1, PD-1/PD-L2, and other co-inhibitory signaling pathways in transplantation. Transpl Int. 21(11): 1015-28.
  • Riley JL.(2009) PD-1 signaling in primary T cells. Immunol Rev. 229(1): 114-25.
  • Epitope mapping reveals the binding mechanism of a functional antibody cross-reactive to both human and murine programmed death 1
    Author
    Li, D;Xu, J;Wang, Z;Gong, Z;Liu, J;Zheng, Y;Li, J;Li, J;
    Year
    2017
    Journal
    mAbs
    Application
    binding
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