Mouse S100A4 HEK293 Overexpression Lysate

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Mouse S100A4 HEK293 Overexpression Lysate: 製品の情報

製品の説明
This Mouse S100A4 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of S100A4 protein (Cat: 50484-MNCH) from the overexpression lysate was verified.
発現ホスト
HEK293 Cells
Mouse
タンパク質構築情報
A DNA sequence encoding the mouse S100A4 (P07091)(Ala2-Lys101) was expressed and purified with two additional amino acids (Gly & Pro) at the N-terminus.
分子量
The recombinant mouse S100A4 consists of 102 amino acids and predicts a molecular mass of 11.7 KDa. It migrates as an approximately 12 KDa band in SDS-PAGE under reducing conditions.

Mouse S100A4 HEK293 Overexpression Lysate: 用法

調製方法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
溶解バッファー
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
おすすめの用法
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
バッファー
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
安定性 & 保存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
アプリケーション
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Mouse S100A4 HEK293 Overexpression Lysate: 別名

Mouse 18A2 Overexpression Lysate; Mouse 42a Overexpression Lysate; Mouse Capl Overexpression Lysate; Mouse FSp1 Overexpression Lysate; Mouse metastasin Overexpression Lysate; Mouse Mts1 Overexpression Lysate; Mouse PeL98 Overexpression Lysate; Mouse pk9a Overexpression Lysate

S100A4 背景情報

S1A4, also known as metastasis-associated protein Mtsl, belongs to the family of small calcium-binding S1 proteins containing two EF-hand calcium-binding motifs. In humans at least 2 S1 family members that are distributed tissue specifically have been identified, and are involved in a number of cellular processes as transducers of calcium signal. S1A4 is a symmetric homodimer, and undergoes a relatively large conformational change upon the typical EF-hand binding calcium, which is necessary for S1A4 to interact with its protein targets and generate biological effects. It can bind the already known targets p53, F-actin, liprin β, myosin heavy chain II, and prevent their phosphorylation and multimerization. It has been demonstrated that S1A4 is directly involved in tumor metastasis including cell motility, invasion, apoptosis, angiogenesis and differentiation, and appears to be a metastasis factor and a molecular marker for clinical prognosis. Multiple alternatively spliced variants encoding the same protein have been identified.
完全な名称
S100 calcium binding protein A4
参考文献
  • Ambartsumian N. et al., 1995, Gene. 159: 125-30.
  • Marenholz I. et al., 2004, Biochem Biophys Res Commun. 322: 1111-22.
  • Helfman DM. et al., 2005, Br J Cancer. 92: 1955-8.
  • Saleem M. et al., 2006, Proc Natl Acad Sci. 103: 14825-30.
  • Boye K. et al., 2008, Int J Cancer. 123: 1301-10.
  • Garrett SC. et al., 2006, J Biol Chem. 281: 677-80.
  • Kriajevska M. et al., 2002, J Biol Chem. 277: 5299-335.
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