PD-L1 cDNA ORF Clone, Mouse, C-HA tag

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PD-L1 cDNA ORF Clone, Mouse, C-HA tag: 製品情報

遺伝子
Mouse
NCBI 参考シーケンス番号
遺伝子の長さ
873 bp
シーケンスの特徴
Identical with the Gene Bank Ref. ID sequence.
製品の特徴
Full length Clone DNA of Mouse CD274 antigen with C terminal HA tag.
プラスミド
プロモーター
Enhanced CMV promoter
ベクター
制限部位
KpnI + XbaI (6kb + 0.92kb)
タグシーケンス
HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
シークエンシングプライマー
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
品質管理
The plasmid is confirmed by full-length sequencing.
スクリーニング
細菌スクリーニング抵抗力
Kanamycin
細胞スクリーニング抵抗力
Hygromycin
アプリケーション
Stable or Transient mammalian expression
保存 & 配送
配送方法
Each tube contains lyophilized plasmid.
保存条件
The lyophilized plasmid can be stored at ambient temperature for three months.

PD-L1 cDNA ORF ヌクレオチド配列およびアミノ酸配列に関する情報

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

PD-L1 cDNA ORF Clone, Mouse, C-HA tag: 検証済の画像

The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF02). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-HA Tag monoclonal antibody (100028-MM10) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.

PD-L1 cDNA ORF Clone, Mouse, C-HA tag: 別名

A530045L16Rik cDNA ORF Clone, Mouse; B7h1 cDNA ORF Clone, Mouse; Pdcd1l1 cDNA ORF Clone, Mouse; Pdcd1lg1 cDNA ORF Clone, Mouse; Pdl1 cDNA ORF Clone, Mouse

PD-L1 背景情報

Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules and this protein contains one V-like and one C-like Ig domain within the extracellular domain, and together with PD-L2, are two ligands for PD1 which belongs to the CD28/CTLA4 family expressed on activated lymphoid cells. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.
完全な名称
CD274 molecule
参考文献
  • Iwai Y, et al. (2002) Involvement of PD-L1 on tumor cells in the escape from host immune system and tumor immunotherapy by PD-L1 blockade. Proc Natl Acad Sci U S A. 99(19): 12293-7.
  • Ghebeh H, et al. (2006) The B7-H1 (PD-L1) T lymphocyte-inhibitory molecule is expressed in breast cancer patients with infiltrating ductal carcinoma: correlation with important high-risk prognostic factors. Neoplasia. 8(3): 190-8.
  • Salih HR, et al. (2006) The role of leukemia-derived B7-H1 (PD-L1) in tumor-T-cell interactions in humans. Exp Hematol. 34(7): 888-94.
  • Wilcox RA, et al. (2009) B7-H1 (PD-L1, CD274) suppresses host immunity in T-cell lymphoproliferative disorders. Blood. 114(10): 2149-58.
  • Ruggiero A, et al. (2009) Crystal structure of PD-L1, a ribosome inactivating protein from Phytolacca dioica L. leaves with the property to induce DNA cleavage. Biopolymers. 91(12): 1135-42.

PD-L1 cDNA ORFクローン, Rabbit, 関連製品

  • Targeting the upstream transcriptional activator of PD-L1 as an alternative strategy in melanoma therapy
    Author
    Zhu, B;Tang, L;Chen, S;Yin, C;Peng, S;Li, X;Liu, T;Liu, W;Han, C;Stawski, L;Xu, ZX;Zhou, G;Chen, X;Gao, X;Goding, CR;Xu, N;Cui, R;Cao, P;
    Year
    2018
    Journal
    Oncogene
    Application
    expression
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