p38 Protein, Human, Recombinant (His Tag)

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p38 Protein, Human, Recombinant (His Tag): 製品情報

純度
> 90 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
活性
Kinase activity untested
タンパク質の構築
A DNA sequence encoding the human MAPK14 isoform 2 (NP_620581.1) (Met1-Ser360) was fused with a polyhistide tag at the N-terminus.
NP No.
発現ホスト
Baculovirus-Insect Cells
Human
予測N末端
His
分子量
The recombinant human MAPK14 consists of 378 amino acids and has a calculated molecular mass of 43.7 kDa. The recombinant protein migrates as an approximately 43 kDa band in SDS-PAGE under reducing conditions.
バッファー
Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
配送
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Shipment made at ambient temperature may seriously affect the activity of the ordered products.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

p38 Protein, Human, Recombinant (His Tag): 画像

p38 Protein, Human, Recombinant (His Tag): 別名

CSBP Protein, Human; CSBP1 Protein, Human; CSBP2 Protein, Human; CSPB1 Protein, Human; EXIP Protein, Human; Mxi2 Protein, Human; p38 Protein, Human; p38ALPHA Protein, Human; PRKM14 Protein, Human; PRKM15 Protein, Human; RK Protein, Human; SAPK2A Protein, Human

p38 背景情報

MAPK14 contains 1 protein kinase domain and belongs to the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. MAPK14 can be detected in brain, heart, placenta, pancreas and skeletal muscle and it is expressed to a lesser extent in lung, liver and kidney. MAPK14 is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with MAPK14. The substrates of p38 alpha include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of p38 alpha in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. In respond to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide, MAPK14 phosphorylates a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. MAPK14 plays a critical role in the production of some cytokines, for example IL-6. It may play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2.
完全な名称
mitogen-activated protein kinase 14
研究分野
参考文献
  • Luo X, et al. (2011) Study on p38 mitogen activated protein kinase in vascular endothelial cells dysfunction in preeclampsia. Zhonghua Fu Chan Ke Za Zhi. 46(1):36-40.
  • Park CH, et al. (2011) Epidermal growth factor-induced matrix metalloproteinase-1 expression is negatively regulated by p38 MAPK in human skin fibroblasts. J Dermatol Sci. 64(2):134-41.
  • Lee JY, et al. (2011) Curcumin induces EGFR degradation in lung adenocarcinoma and modulates p38 activation in intestine: the versatile adjuvant for gefitinib therapy. PLoS One. 6(8):e23756.
  • Riis JL, et al. (2011) CCL27 expression is regulated by both p38 MAPK and IKKβ signalling pathways. Cytokine. 56(3):699-707.
  • Host ICAMs play a role in cell invasion by Mycobacterium tuberculosis and Plasmodium falciparum
    Author
    Bhalla, K;Chugh, M;Mehrotra, S;Rathore, S;Tousif, S;Prakash Dwivedi, V;Prakash, P;Kumar Samuchiwal, S;Kumar, S;Kumar Singh, D;Ghanwat, S;Kumar, D;Das, G;Mohmmed, A;Malhotra, P;Ranganathan, A;
    Year
    2015
    Journal
    Nat Commun
    Application
    substrate
  • Surface plasmon resonance imaging validation of small molecule drugs binding on target protein microarrays
    Author
    Zhou, W;Yang, M;Li, S;Zhu, J;
    Year
    2018
    Journal
    Applied Surface Science
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