IGFBP-6 Protein, Mouse, Recombinant (His Tag)

1/1
Price:
Size:
Number:

IGFBP-6 Protein, Mouse, Recombinant (His Tag): 製品情報

純度
> 92 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
活性
Measured by its ability to inhibit the biological activity of IGFII on MCF7 human breast adenocarcinoma cells (Karey, K.P. et al. (1988) Cancer Research 48:4083.) . The ED50 for this effect is typically 1-4 μg/ml in the presence of 20 ng/mL mouse IGFII.
タンパク質の構築
A DNA sequence encoding the mouse IGFBP6 (NP_032370.2) (Met 1-Gly 238) was expressed, with a polyhistidine tag at the C-terminus.
NP No.
発現ホスト
HEK293 Cells
Mouse
予測N末端
Gln 20
分子量
The recombinant mouse IGFBP6 consists of 224 amino acids and has a predicted molecular mass of 24 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmIGFBP6 is approximately 30 kDa due to glycosylation.
バッファー
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IGFBP-6 Protein, Mouse, Recombinant (His Tag): 画像

IGFBP-6 Protein, Mouse, Recombinant (His Tag): 別名

IGFBP-6 Protein, Mouse

IGFBP-6 背景情報

Insulin-like growth factor binding protein 6 (IGFBP6) is a 24-kDa protein that binds insulin-like growth factor 1 (IGF-1) and IGF-2 with high affinity and inhibits IGF action in vitro. The Insulin-like growth factor-binding protein also known as IGFBP serves as a carrier protein for Insulin-like growth factor 1. IGFBPs are clearly distinct but are sharing regions with strong homology. All members of the IGFBP family bind IGF-I and IGF-II with about equal affinity. Insulin-like growth factor (IGF) binding proteins (IGFBPs) have been shown to either inhibit or enhance the action of IGF, or act in an IGF-independent manner in the prostate. IGF-binding protein-4 (IGFBP-4) inhibits IGF-I action in vitro and is the most abundant IGFBP in the rodent arterial wall. IGFBP6 is directly downregulated by the beta-catenin/TCF complex in desmoid tumors, and imply a role for the IGF axis in the proliferation of desmoid tumors. There is mounting evidence that the structure of the IGFBP proteins plays a key role in the regulation of IGF bioavailability, by modulating its molecular size, capillary membrane permeability, target tissue specificity, cell membrane adherence and IGF affinity.
完全な名称
insulin-like growth factor binding protein 6
参考文献
  • Denys H, et al. (2004) Identification of IGFBP-6 as a significantly downregulated gene by beta-catenin in desmoid tumors. Oncogene. 23(3): 654-64.
  • Bach LA. Insulin-like growth factor binding protein-6: the "forgotten" binding protein? Horm Metab Res. 31(2-3): 226-34.
  • Bach LA. IGFBP-6 five years on; not so 'forgotten'? Growth Horm IGF Res. 15(3): 185-92.
カートに追加しました カートに追加しませんでした カートを更新しています。少々お待ちください