eIF2A/eIF2 alpha cDNA ORF Clone, Human, C-DDK (Flag®) tag

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eIF2A/eIF2 alpha cDNA ORF Clone, Human, C-DDK (Flag®) tag: 製品情報

遺伝子
Human
NCBI 参考シーケンス番号
遺伝子の長さ
1758 bp
製品の特徴
Full length Clone DNA of Human eukaryotic translation initiation factor 2A, 65kDa (EIF2A) with C terminal Flag tag.
プラスミド
プロモーター
Enhanced CMV promoter
ベクター
タグシーケンス
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
シークエンシングプライマー
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
品質管理
The plasmid is confirmed by full-length sequencing.
スクリーニング
細菌スクリーニング抵抗力
Kanamycin
細胞スクリーニング抵抗力
Hygromycin
アプリケーション
Stable or Transient mammalian expression
保存 & 配送
配送方法
Each tube contains lyophilized plasmid.
保存条件
The lyophilized plasmid can be stored at ambient temperature for three months.

eIF2A/eIF2 alpha cDNA ORF Clone, Human, C-DDK (Flag®) tag: 検証済の画像

The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF02). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.

eIF2A/eIF2 alpha cDNA ORF Clone, Human, C-DDK (Flag®) tag: 別名

CDA02 cDNA ORF Clone, Human; EIF-2A cDNA ORF Clone, Human; MST089 cDNA ORF Clone, Human; MSTP004 cDNA ORF Clone, Human; MSTP089 cDNA ORF Clone, Human

eIF2A/eIF2 alpha 背景情報

完全な名称
eukaryotic translation initiation factor 2A, 65kDa
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