SUMO2 cDNA ORF Clone, Human, C-DDK (Flag®) tag

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SUMO2 cDNA ORF Clone, Human, C-DDK (Flag®) tag: 製品情報

遺伝子
Human
NCBI 参考シーケンス番号
遺伝子の長さ
288 bp
シーケンスの特徴
Identical with the Gene Bank Ref. ID sequence.
製品の特徴
Full length Clone DNA of Human SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae) with C terminal Flag tag.
プラスミド
プロモーター
Enhanced CMV promoter
ベクター
制限部位
KpnI + XbaI (6kb + 0.34kb)
タグシーケンス
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
シークエンシングプライマー
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
品質管理
The plasmid is confirmed by full-length sequencing.
スクリーニング
細菌スクリーニング抵抗力
Kanamycin
細胞スクリーニング抵抗力
Hygromycin
アプリケーション
Stable or Transient mammalian expression
保存 & 配送
配送方法
Each tube contains lyophilized plasmid.
保存条件
The lyophilized plasmid can be stored at ambient temperature for three months.

SUMO2 cDNA ORF ヌクレオチド配列およびアミノ酸配列に関する情報

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

SUMO2 cDNA ORF Clone, Human, C-DDK (Flag®) tag: 検証済の画像

The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF02). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.

SUMO2 cDNA ORF Clone, Human, C-DDK (Flag®) tag: 別名

HSMT3 cDNA ORF Clone, Human; Smt3A cDNA ORF Clone, Human; SMT3B cDNA ORF Clone, Human; SMT3H2 cDNA ORF Clone, Human; SUMO3 cDNA ORF Clone, Human

SUMO2 背景情報

SUMO2 gene silencing promotes proliferation, and inhibits the apoptosis and senescence of NPCs in rats with IDD through the down-regulation of the p53 signaling pathway. Thus, SUMO2 is a potential target in the treatment of Intervertebral disc degeneration (IDD). The small ubiquitinrelated modifier protein (SUMO) functions as a posttranslational modification system and SUMO2/3 subtypes are often activated in early stress. SUMO2 as a novel activator of Cn-NFAT signaling in cardiomyocytes.
完全な名称
small ubiquitin-like modifier 2
  • TRIB3 Promotes APL Progression through Stabilization of the Oncoprotein PML-RARα and Inhibition of p53-Mediated Senescence
    Author
    Li, K;Wang, F;Cao, WB;Lv, XX;Hua, F;Cui, B;Yu, JJ;Zhang, XW;Shang, S;Liu, SS;Yu, JM;Han, MZ;Huang, B;Zhang, TT;Li, X;Jiang, JD;Hu, ZW;
    Year
    2017
    Journal
    Cancer Cell
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