ORP150 cDNA ORF Clone, Human, C-GFPSpark® tag

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ORP150 cDNA ORF Clone, Human, C-GFPSpark® tag: 製品情報

遺伝子
Human
NCBI 参考シーケンス番号
遺伝子の長さ
3000 bp
シーケンスの特徴
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 2235A>G not causing the amino acid variation.
製品の特徴
Full length Clone DNA of Human hypoxia up-regulated 1 with C terminal GFPSpark tag.
プラスミド
プロモーター
Enhanced CMV promoter
ベクター
制限部位
HindIII + NotI (6kb + 3.73kb)
タグシーケンス
GFPSpark: GTGAGCAAGGGC……GAGCTGTACAAG
シークエンシングプライマー
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
品質管理
The plasmid is confirmed by full-length sequencing.
スクリーニング
細菌スクリーニング抵抗力
Kanamycin
細胞スクリーニング抵抗力
Hygromycin
アプリケーション
Stable or Transient mammalian expression
保存 & 配送
配送方法
Each tube contains lyophilized plasmid.
保存条件
The lyophilized plasmid can be stored at ambient temperature for three months.

ORP150 cDNA ORF ヌクレオチド配列およびアミノ酸配列に関する情報

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

ORP150 cDNA ORF Clone, Human, C-GFPSpark® tag: 検証済の画像

The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat#STF01) transiently. After 48 h, the fluorescent signals can be detected by fluorescence microscope. Green excitation 475/40nm, emission 535/45nm. Red excitation 560/55nm, emission 645/45nm. Orange excitation 525/45nm, emission 595/60nm.

ORP150 cDNA ORF Clone, Human, C-GFPSpark® tag: 別名

GRP-170 cDNA ORF Clone, Human; Grp170 cDNA ORF Clone, Human; HSP12A cDNA ORF Clone, Human; ORP-150 cDNA ORF Clone, Human; ORP150 cDNA ORF Clone, Human

ORP150 背景情報

Hypoxia up-regulated protein 1, also known as 15 kDa oxygen-regulated protein, 17 kDa glucose-regulated protein, ORP-15, GRP-17 and HYOU1, is a member of theheat shock protein 7 family. Seven members from four different heat shock protein (HSP) families were identified including HYOU1 (ORP15), HSPC1 (HSP86), HSPA5 (Bip), HSPD1 (HSP6), and several isoforms of the two testis-specific HSP7 chaperones HSPA2 and HSPA1L. HYOU1 is highly expressed in tissues that contain well-developed endoplasmic reticulum and synthesize large amounts of secretory proteins. It is highly expressed in liver and pancreas. HYOU1 is also expressed in macrophages within aortic atherosclerotic plaques, and in breast cancers. HYOU1 has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. It may play a role as a molecular chaperone and participate in protein folding. Suppression of HYOU1 is associated with accelerated apoptosis. It is suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness.
完全な名称
hypoxia up-regulated 1
参考文献
  • Ikeda J., et al.,(1997), Cloning and expression of cDNA encoding the human 150 kDa oxygen-regulated protein, ORP150. Biochem. Biophys. Res. Commun. 230:94-99.
  • Kaneda S., et al., (2000), Production of three distinct mRNAs of 150 kDa oxygen-regulated protein (ORP150) by alternative promoters: preferential induction of one species under stress conditions.J. Biochem. 128:529-538.
  • Takeuchi S., (2006), Molecular cloning, sequence, function and structural basis of human heart 150 kDa oxygen-regulated protein, an ER chaperone.Protein J. 25:517-528.
  • Protectin DX ameliorates hepatic steatosis by suppression of endoplasmic reticulum stress via AMPK-induced ORP150 expression
    Author
    Jung, TW;Kyung, EJ;Kim, HC;Shin, YK;Lee, SH;Park, ES;Hacimuftuoglu, A;Abd El-Aty, AM;Jeong, JH;
    Year
    2018
    Journal
    J. Pharmacol. Exp. Ther.
    Application
    expression
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