EGF Protein, Human, Recombinant

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EGF Protein, Human, Recombinant: 製品情報

純度
> 95 % as determined by SDS-PAGE
内毒素
< 0.01 EU per μg of the protein as determined by the LAL method
活性
Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The EC50 for this effect is typically 0.1-0.5 ng/mL.
The specific activity of Recombinant Human EGF is approximately 1.9×10E3 IU/μg, which is calibrated against human EGF WHO International Standard (NIBSC code: 91/530).
タンパク質の構築
A DNA sequence encoding the mature form of human EGF (NP_001954.2) (Asn 971-Arg 1023) was expressed and purified with an initial Met at the N-terminus.
NP No.
発現ホスト
E. coli
Human
予測N末端
Met
分子量
The recombinant human EGF consists of 54 amino acids and has a calculated molecular mass of 6.3 kDa.
バッファー
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

EGF Protein, Human, Recombinant: 画像

Human EGF / Epidermal Growth Factor Protein
Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The EC50 for this effect is typically 0.1-0.5 ng/mL. The specific activity of Recombinant Human EGF is approximately 1.9×10E3 IU/μg, which is calibrated against human EGF WHO International Standard (NIBSC code: 91/530).

EGF Protein, Human, Recombinant: 別名

Epidermal Growth Factor Protein, Human; HOMG4 Protein, Human; URG Protein, Human

EGF 背景情報

EGF is the founding member of the EGF-family of proteins. Members of this protein family have highly similar structural and functional characteristics. EGF contains 9 EGF-like domains and 9 LDL-receptor class B repeats. Human EGF is a 645-Da protein with 53 amino acid residues and three intramolecular disulfide bonds. As a low-molecular-weight polypeptide, EGF was first purified from the mouse submandibular gland, but since then it was found in many human tissues including submandibular gland, parotid gland. It can also be found in human platelets, macrophages, urine, saliva, milk, and plasma. EGF is a growth factor that stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. It results in cellular proliferation, differentiation, and survival. Salivary EGF, which seems also regulated by dietary inorganic iodine, also plays an important physiological role in the maintenance of oro-esophageal and gastric tissue integrity. EGF acts by binding with high affinity to epidermal growth factor receptor on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. The tyrosine kinase activity, in turn, initiates a signal transduction cascade that results in a variety of biochemical changes within the cell - a rise in intracellular calcium levels, increased glycolysis and protein synthesis, and increases in the expression of certain genes including the gene for EGFR - that ultimately lead to DNA synthesis and cell proliferation.
完全な名称
epidermal growth factor
参考文献
  • Chen JX, et al. (2011) Involvement of c-Src/STAT3 signal in EGF-induced proliferation of rat spermatogonial stem cells. Mol Cell Biochem. 358(1-2):67-73.
  • Guo Y, et al. (2012) Correlations among ERCC1, XPB, UBE2I, EGF, TAL2 and ILF3 revealed by gene signatures of histological subtypes of patients with epithelial ovarian cancer. Oncol Rep. 27(1):286-92.
  • Kim S, et al. (2012) Smad7 acts as a negative regulator of the epidermal growth factor (EGF) signaling pathway in breast cancer cells. Cancer Lett. 314(2):147-54.
  • Chatterton RT Jr, et al. (2010) Breast ductal lavage for assessment of breast cancer biomarkers. Horm Cancer. 1(4):197-204.
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