Anti-Hsp27 / HSPB1 Antibody

1/2
Price:
Size:
Number:

Anti-Hsp27 / HSPB1 Antibody (Rabbit Monoclonal 抗体) の製品情報

製品名
Anti-Hsp27 / HSPB1 Antibody
検証済のアプリケーション
WB,ELISA,ELISA(Cap),IP
交差反応
Reacts with: Human
特異性
Human Hsp27 / HSPB1
免疫原
Recombinant Human HSP27 protein (Catalog#10351-H08E)
調製
This antibody was obtained from a rabbit immunized with purified, recombinant Human HSP27 (rh HSP27; Catalog#10351-H08E; NP_001532.1; Met 1-Lys 205) .
ソース
Monoclonal Rabbit IgG Clone #017
精製
Protein A
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Hsp27 / HSPB1 Antibody (Rabbit Monoclonal 抗体) 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:500-1:2000
ELISA 1:5000-1:10000
IP 1-4 μL/mg of lysate
ELISA(Cap) 1:250-1:2000
Notes
ELISA(Cap): This antibody will detect Human HSPB1 / HSP 27 in ELISA pair set (Catalog: # SEK10351). In a sandwich ELISA, it can be used as capture antibody when paired with (Catalog: # 10351-MM01).
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Hsp27 / HSPB1 Antibody (Rabbit Monoclonal 抗体) の画像

HSP27 was immunoprecipitated using:

Lane A:0.5 mg Caco-2 Whole Cell Lysate

2 µL anti-HSP27 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-HSP27 rabbit monoclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 25 kDa

Observed band size: 25 kDa

Anti-HSP27 rabbit monoclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:23 kDa

Observed band size:26 kDa

Anti-Hsp27 / HSPB1 Antibody: 別名

Anti-CMT2F Antibody; Anti-HEL-S-102 Antibody; Anti-HMN2B Antibody; Anti-HS.76067 Antibody; Anti-Hsp25 Antibody; Anti-HSP27 Antibody; Anti-HSP28 Antibody; Anti-SRP27 Antibody

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

カートに追加しました カートに追加しませんでした カートを更新しています。少々お待ちください