c-MET Protein, Human, Recombinant (His & Fc Tag)

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c-MET Protein, Human, Recombinant (His & Fc Tag): 製品情報

純度
> 95 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
活性
Measured by its binding ability in a functional ELISA. Immobilized recombinant human HGF at 1 μg/ml (100ul/well) can bind Human c-MET / HGFR with a linear range of 0.31-160ng/ml. Scatchard analysis showed the affinity constant (Kd) of human HGF bound to human c-MET / HGFR was 0.52 nM .
タンパク質の構築
A DNA sequence encoding the extracellular domain (Met 1-Thr 932) of human c-Met (NP_000236) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
NP No.
発現ホスト
HEK293 Cells
Human
予測N末端
Glu 25
分子量
The mature recombinant human c-Met/Fc is a disulfide-linked tetramer composed of two proteolytically cleaved α and β subunits. Each α and β together with the C-terminal Fc tag consists of 1155 amino acids and has a predicted molecular mass of 129.5 (α =32.5 + Fc tagged β=97) kDa. The rh c-MET/Fc heterodimer thus migrates with apparent molecular mass of approximately 45 kDa and 120 kDa respectively in SDS-PAGE under reducing conditions due to glycosylation.
バッファー
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

c-MET Protein, Human, Recombinant (His & Fc Tag): 画像

Measured by its binding ability in a functional ELISA. Immobilized recombinant human HGF at 1 μg/ml (100ul/well) can bind Human c-MET / HGFR with a linear range of 0.31-160ng/ml. Scatchard analysis showed the affinity constant (Kd) of human HGF bound to human c-MET / HGFR was 0.52 nM .

c-MET Protein, Human, Recombinant (His & Fc Tag): 別名

AUTS9 Protein, Human; c-Met Protein, Human; DFNB97 Protein, Human; HGFR Protein, Human; RCCP2 Protein, Human

c-MET 背景情報

Hepatocyte growth factor receptor (HGFR), also known as c-Met or mesenchymal-epithelial transition factor (MET), is a receptor tyrosine kinase (RTK) that has been shown to be overexpressed and/or mutated in a variety of malignancies. HGFR protein is produced as a single-chain precursor, and HGF is the only known ligand. Normal HGF/HGFR signaling is essential for embryonic development, tissue repair or wound healing, whereas aberrantly active HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes. HGFR protein is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin. Preclinical studies suggest that targeting aberrant HGFR signaling could be an attractive therapy in cancer.
完全な名称
MET proto-oncogene, receptor tyrosine kinase
参考文献
  • McGill GG, et al. (2006) c-Met expression is regulated by Mitf in the melanocyte lineage. J Biol Chem. 281(15): 10365-73.
  • Garcia S, et al. (2007) c-Met overexpression in inflammatory breast carcinomas: automated quantification on tissue microarrays. British journal of cancer. 96(2): 329-35.
  • Socoteanu MP, et al. (2008) c-Met targeted therapy of cholangiocarcinoma. World J Gastroenterol. 14(19): 2990-4.
  • Kong DS, et al. (2009) Prognostic significance of c-Met expression in glioblastomas. Cancer. 115(1): 140-8.
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