Anti-Granzyme B Antibody

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Anti-Granzyme B Antibody (Mouse Monoclonal 抗体) の製品情報

製品名
Anti-Granzyme B Antibody
検証済のアプリケーション
ELISA,IHC-P
交差反応
Reacts with: Human
特異性
Human Granzyme B
免疫原
Recombinant Human Granzyme B protein (Catalog#10345-H08H)
調製
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Granzyme B (rhGranzyme B; Catalog#10345-H08H; NP_004122.1; Met 1-Tyr 247). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
ソース
Monoclonal Mouse IgG2b Clone #2F5F2D10
精製
Protein A
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Granzyme B Antibody (Mouse Monoclonal 抗体) 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
ELISA 1:1000-1:2000
IHC-P 1:50-1:200
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Granzyme B Antibody (Mouse Monoclonal 抗体) の画像

Immunochemical staining of human Granzyme B in human tonsil with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections).
Immunochemical staining of human Granzyme B in human spleen with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections).
Immunochemical staining of human Granzyme B in human lymph node with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections).

Anti-Granzyme B Antibody: 別名

Anti-CCPI Antibody; Anti-CGL-1 Antibody; Anti-CGL1 Antibody; Anti-CSP-B Antibody; Anti-CSPB Antibody; Anti-CTLA1 Antibody; Anti-CTSGL1 Antibody; Anti-HLP Antibody; Anti-SECT Antibody

Granzyme B 背景情報

Granzyme B, also known as GZMB, is the most prominent member of the granzyme family of cell death-inducing serine proteases expressed in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. Granzyme B enters the target cells depending on another membrane-binding granule protein, perforin, results in the activation of effector caspases and mitochondrial depolarization through caspase-dependent and -independent pathways, and consequently induces rapid cell apoptosis. Over 3 substrates of GZMB have been identified including the key substrate caspase-3, ICAD and Bid. GZMB is suggested to protect the host by lysing cells bearing on their surface 'nonself' antigens such as bacterial and viral infected-cells and tumor cells, and accordingly plays an essential role in immunosurveillance.
完全な名称
granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
参考文献
  • Schmid J., et al.,(1987), Induction of mRNA for a serine protease and a beta-thromboglobulin-like protein in mitogen-stimulated human leukocytes. J. Immunol. 139:250-256.
  • Caputo A., et al., (1988), Structure and differential mechanisms of regulation of expression of a serine esterase gene in activated human T lymphocytes.J. Biol. Chem. 263:6363-6369.
  • Trapani J.A., et al.,(1988), Molecular cloning of an inducible serine esterase gene from human cytotoxic lymphocytes.Proc. Natl. Acad. Sci. U.S.A. 85:6924-6928.

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