Human GOLPH2 / GOLM1 No cross-reactivity in ELISA with Human cell lysate (293 cell line)
Recombinant Human GOLM1 / GP73 protein (Catalog#13066-H08H)
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human GOLM1 / GP73 (rh GOLM1 / GP73; Catalog#13066-H08H; Q8NBJ4-1; Val 40-Leu 401). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Monoclonal Mouse IgG1 Clone #9C2D3
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunochemical staining of human GOLM1 in human liver with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections). Positive staining was localized to cytoplasm of hepatocyte and interlobular bile duct.
Immunochemical staining of human GOLM1 in human endometrium with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections). Positive staining was localized to cytoplasm of uterine gland.
Golgi membrane protein 1, also known as Golgi membrane protein GP73, Golgi phosphoprotein 2 and GOLM1, is a protein which belongs to theGOLM1 / CASC4 family. GOLM1 is widely expressed. It is highly expressed in colon, prostate, trachea and stomach. It is expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. It is predominantly expressed by cells of the epithelial lineage. GOLM1 is expressed at low level in normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression of GOLM1 does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), GOLM1 is strongly expressed in hepatocyte-derived syncitial giant cells. GOLM1 is constitutively expressed by biliary epithelial cells but not by hepatocytes.
Kladney R.D., et al.,(2000), GP73, a novel Golgi-localized protein upregulated by viral infection. Gene 249:53-65.
Clark H.F., et al., (2003), The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.Genome Res. 13:2265-2270.
Puri S., et al.,(2002), Cycling of early Golgi proteins via the cell surface and endosomes upon lumenal pH disruption.Traffic 3:641-653.