Anti-E-Cadherin Antibody (PE)

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Anti-E-Cadherin Antibody (PE) (Mouse Monoclonal 抗体) の製品情報

製品名
Anti-E-Cadherin Antibody (PE)
検証済のアプリケーション
FCM,ICC/IF
交差反応
Reacts with: Human
特異性
Human E-Cadherin
免疫原
Recombinant Human Cadherin-1 / CDH1 / CD324 / E-cadherin protein (Catalog#10204-H08H)
調製
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Cadherin-1 / CDH1 / CD324 / E-cadherin (rh Cadherin-1 / CDH1 / CD324 / E-cadherin; Catalog#10204-H08H; NP_004351.1; Met 1-Ile 707) and conjugated with PE under optimum conditions, the unreacted PE was removed.
ソース
Monoclonal Mouse IgG2a Clone #08
精製
Protein A
緩衝液
Aqueous solution containing 0.5% BSA and 0.09% sodium azide
標識物
PE
濃度
5 μl/Test, 0.1 mg/ml
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Anti-E-Cadherin Antibody (PE) (Mouse Monoclonal 抗体) 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
ICC-IF 5-20 μg/mL
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-E-Cadherin Antibody (PE) (Mouse Monoclonal 抗体) の画像

Profile of anti-E-cad (CD324) reactivity on MCF-7 cells analyzed by flow cytometry.
Immunofluorescence staining of Human E-CAD in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human MCF7 monoclonal antibody (PE-conjugated, 10 µg/ml) at 37℃ 1 hour, and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.

Anti-E-Cadherin Antibody (PE): 別名

Anti-Arc-1 Antibody; Anti-CD324 Antibody; Anti-CDH1 Antibody; Anti-CDHE Antibody; Anti-E-cad Antibody; Anti-E-Cadherin Antibody; Anti-ECAD Antibody; Anti-LCAM Antibody; Anti-UVO Antibody

E-Cadherin 背景情報

Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is a calcium-dependent cell adhesion molecule the intact function of which is crucial for the establishment and maintenance of epithelial tissue polarity and structural integrity. Mutations in CDH1 occur in diffuse type gastric cancer, lobular breast cancer, and endometrial cancer. In human cancers, partial or complete loss of E-cadherin expression correlates with malignancy. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas.
完全な名称
cadherin 1, type 1, E-cadherin (epithelial)
参考文献
  • Wang HD, et al. (2004) CDH1 germline mutation in hereditary gastric carcinoma. World J Gastroenterol. 10(21): 3088-93.
  • Masterson J, et al. (2007) Posttranslational truncation of E-cadherin and significance for tumour progression. Cells Tissues Organs. 185(1-3): 175-9.
  • Mrgineanu E, et al. (2008) Correlation between E-cadherin abnormal expressions in different types of cancer and the process of metastasis. Rev Med Chir Soc Med Nat Iasi. 112(2): 432-6.

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