Anti-ICAM-1 Antibody

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Anti-ICAM-1 Antibody (Rabbit Monoclonal 抗体) の製品情報

製品名
Anti-ICAM-1 Antibody
検証済のアプリケーション
ELISA,ELISA(Cap),IHC-P
交差反応
Reacts with: Mouse
特異性
Mouse ICAM-1
No cross-reactivity in ELISA with
Human ICAM1
Human CD31
Human ICAM3
Human ICAM2
Mouse VCAM1
免疫原
Recombinant Mouse CD54 / ICAM1 protein (Catalog#50440-M08H)
調製
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD54 / ICAM1 (rM CD54 / ICAM1; Catalog#50440-M08H; NP_034623.1; Met 1-Asn 485).
ソース
Monoclonal Rabbit IgG Clone #020
精製
Protein A
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-ICAM-1 Antibody (Rabbit Monoclonal 抗体) 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
ELISA 1:5000-1:10000
IHC-P 1:100-1:500
ELISA(Cap) 1:250-1:2000
Notes
ELISA(Cap): This antibody will detect Mouse ICAM-1 / CD54 in ELISA pair set (Catalog: # SEK50440). In a sandwich ELISA, it can be used as capture antibody when paired with (Catalog: # 50440-R101).
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-ICAM-1 Antibody (Rabbit Monoclonal 抗体) の画像

Immunochemical staining of mouse ICAM1 in mouse kidney with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to blood vessel.
Immunochemical staining of mouse ICAM1 in mouse brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). The image showing positive staining of choroid plexus.

Anti-ICAM-1 Antibody: 別名

Anti-CD54 Antibody; Anti-Icam-1 Antibody; Anti-Ly-47 Antibody; Anti-MALA-2 Antibody

ICAM-1 背景情報

Intercellular adhesion molecule-1 (ICAM-1, or CD54) is a 9 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and in animal models of atherosclerosis. Additionally, ICAM-1 has been implicated in the progression of autoimmune diseases. ICAM-1 is a ligand for LFA-1(integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM-1 possesses binding sites for a number of immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM-1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease. Previous studies have shown that ICAM-1 is involved in inflammatory reactions and that a defect in ICAM-1 gene inhibits allergic contact hypersensitivity.
完全な名称
intercellular adhesion molecule 1
研究分野
参考文献
  • Xu H, et al. (2001) The role of ICAM-1 molecule in the migration of Langerhans cells in the skin and regional lymph node. Eur J Immunol. 31(10): 3085-93.
  • Terol MJ, et al. (2003) Soluble intercellular adhesion molecule-1 (s-ICAM-1/s-CD54) in diffuse large B-cell lymphoma: association with clinical characteristics and outcome. Ann Oncol. 14(3): 467-74.
  • Mendez MP, et al. (2006) Shedding of soluble ICAM-1 into the alveolar space in murine models of acute lung injury. Am J Physiol Lung Cell Mol Physiol. 290(5): L962-70.
  • Lawson C, et al. (2009) ICAM-1 signaling in endothelial cells. Pharmacol Rep. 61(1): 22-32.
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