Anti-Troponin C Antibody

1/3
Price:
Size:
Number:

Anti-Troponin C Antibody (Rabbit Polyclonal 抗体) の製品情報

製品名
Anti-Troponin C Antibody
検証済のアプリケーション
ELISA,IHC-P,IP
交差反応
Reacts with: Human, Cynomolgus
特異性
Human Troponin C
免疫原
Recombinant Human Troponin C/TNNC1 Protein (Catalog#11011-HNAE)
調製
Produced in rabbits immunized with purified, recombinant Human Troponin C/TNNC1 (rh Troponin C/TNNC1; Catalog#11011-HNAE; NP_003271.1; Met1-Glu161). Troponin C/TNNC1 specific IgG was purified by Human Troponin C/TNNC1 affinity chromatography.
ソース
Polyclonal Rabbit IgG
精製
Protein A & Antigen Affinity
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Troponin C Antibody (Rabbit Polyclonal 抗体) 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
ELISA 1:5000-1:10000
IHC-P 1:500-1:2000
IP IP:5-10 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Troponin C Antibody (Rabbit Polyclonal 抗体) の画像

Immunochemical staining TNNC1 in human skeletal muscle with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining TNNC1 in cynomolgus heart with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.

TNNC1 was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

4 µL anti-TNNC1 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-TNNC1 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 18 kDa

Observed band size :16 kDa

Anti-Troponin C Antibody: 別名

Anti-CMD1Z Antibody; Anti-CMH13 Antibody; Anti-TN-C Antibody; Anti-TNC Antibody; Anti-TNNC Antibody

Troponin C 背景情報

Troponin I, also known as TNNC1, is part of the troponin complex. This complex contains 3 subunits: troponin I (TnI), troponin T (TnT) and troponin C (TnC). Troponin I is the inhibitory subunit, blocking actin-myosin interactions and thereby mediating striated muscle relaxation. It binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place. Because of it myosin cannot bind actin in relaxed muscle. When calcium binds to the Troponin C it causes conformational changes which lead to dislocation of troponin I and finally tropomyosin leaves the binding site for myosin on actin leading to contraction of muscle.
完全な名称
troponin C type 1 (slow)
参考文献
  • Kalaji FR. et al., 2012, Saudi J Kidney Dis Transpl. 23 (5): 939-45.
  • Wijnker PJ. et al., 2013, Am J Physiol Heart Circ Physiol. 304 (2): H260-8.
  • Solaro RJ. et al., 2013, Circ Res. 112 (2): 355-66.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

カートに追加しました カートに追加しませんでした カートを更新しています。少々お待ちください