Anti-PGK2 Antibody

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Anti-PGK2 Antibody (Rabbit Polyclonal 抗体) の製品情報

製品名
Anti-PGK2 Antibody
検証済のアプリケーション
WB,IP
交差反応
Reacts with: Human
Predicted to React with: Cynomolgus (Species predicted to react based on 100% sequence homology)
特異性
Human PGK2
免疫原
A synthetic peptide corresponding to the center region of the Human PGK2
調製
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human PGK2, and purified by antigen affinity chromatography.
ソース
Polyclonal Rabbit IgG
精製
Protein A & Antigen Affinity
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-PGK2 Antibody (Rabbit Polyclonal 抗体) 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:500-1:2000
IP IP:1-5 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-PGK2 Antibody (Rabbit Polyclonal 抗体) の画像

Anti-PGK2 rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lane B: 293T Whole Cell Lysate

Lane C: HeLa Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:45 kDa

Observed band size:45 kDa

PGK2 was immunoprecipitated using:

Lane A:0.5 mg HeLa Whole Cell Lysate

4 µL anti-PGK2 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-PGK2 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 45 kDa

Observed band size :45 kDa

Anti-PGK2 Antibody: 別名

Anti-dJ417L20.2 Antibody; Anti-HEL-S-272 Antibody; Anti-PGKB Antibody; Anti-PGKPS Antibody

PGK2 背景情報

完全な名称
phosphoglycerate kinase 2

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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