Anti-IFNGR1 Antibody

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Anti-IFNGR1 Antibody (Rabbit Polyclonal 抗体) の製品情報

製品名
Anti-IFNGR1 Antibody
検証済のアプリケーション
WB,ELISA,IHC-P,IP
交差反応
Reacts with: Human
特異性
Human IFNGR1
免疫原
Recombinant Human IFNGR1 Protein (Catalog#10338-H08H)
調製
Produced in rabbits immunized with purified, recombinant Human IFNGR1 (rh IFNGR1; Catalog#10338-H08H; NP_000407.1; Met1-Gly245). IFNGR1 specific IgG was purified by Human IFNGR1 affinity chromatography.
ソース
Polyclonal Rabbit IgG
精製
Protein A & Antigen Affinity
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-IFNGR1 Antibody (Rabbit Polyclonal 抗体) 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:500-1:2000
ELISA 1:5000-1:10000
IHC-P 1:500-1:2000
IP IP:5-10 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-IFNGR1 Antibody (Rabbit Polyclonal 抗体) の画像

Anti-IFNgR1 rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:54 kDa

Observed band size:100 kDa

Immunochemical staining of human IFNgR1 in human placenta with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of human IFNgR1 in human kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.

IFNgR1 was immunoprecipitated using:

Lane A:0.5 mg HeLa Whole Cell Lysate

Lane B:0.5 mg 293T Whole Cell Lysate

Lane C:0.5 mg HepG2 Whole Cell Lysate

4 µL anti-IFNgR1 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-IFNgR1 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 54 kDa

Observed band size :100 kDa

Anti-IFNGR1 Antibody: 別名

Anti-CD119 Antibody; Anti-IFNGR Antibody; Anti-IMD27A Antibody; Anti-IMD27B Antibody

IFNGR1 背景情報

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 32 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD119 (cluster of differentiation 119), also known as IFNGR1 ( interferon gamma receptor 1), is part of the heterodimeric gamma interferon receptor which consists of IFNGR1 (CD119) and IFNGR2. The IFNGR1 gene encodes the ligand-binding chain (alpha) of the interteron receptor while IFNGR gene encodes the non-ligand binding partner. The ability of the interferon-γ was achieved through binding to the interferon receptor CD119. After binding, the products of activated T-lymphocytes interferon-γ exerts antiviral activity, growth inhibitory effect, and several immune- regulatory activities on a variety of cell types.
完全な名称
interferon gamma receptor 1
参考文献
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12
  • Novick D, et al. (1987) The human interferon-gamma receptor. The journal of biological chemistry. 262 (18): 8483-7

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