Anti-HA tag Antibody

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Anti-HA tag Antibody (Rabbit Polyclonal 抗体) の製品情報

製品名
Anti-HA tag Antibody
検証済のアプリケーション
WB,IP
特異性
HA tag
免疫原
A synthetic peptide corresponding to the HA-tag sequence (YPYDVPDYA).
調製
Produced in rabbits immunized with a purified synthetic peptide corresponding to the HA-tag sequence. HA-tag specific IgG was purified by HA-tag affinity chromatography.
ソース
Polyclonal Rabbit IgG
精製
Protein A & Antigen Affinity
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-HA tag Antibody (Rabbit Polyclonal 抗体) 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:500-1:1000
IP 0.2-1 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-HA tag Antibody (Rabbit Polyclonal 抗体) の画像

Anti-HA-tag rabbit polyclonal antibody at 1:500 dilution

Lane A: NIH-3T3 whole cell lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:12 kDa

Observed band size:12 kDa

(We are unsure as to the identity of these extra bands.)

HA tag was immunoprecipitated using:

Lane A:0.5 mg HA-mFABP4-myc transfected 293 Whole Cell Lysate

Lane B:0.5 mg myc-mFABP4-HA transfected 293 Whole Cell Lysate

0.5 µL anti-HA tag rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-HA tag rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 14 kDa

Observed band size: 14 kDa

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