Anti-Galectin 7 Antibody

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Anti-Galectin 7 Antibody (Rabbit Polyclonal 抗体) の製品情報

製品名
Anti-Galectin 7 Antibody
検証済のアプリケーション
WB,IHC-P,ICC/IF
交差反応
Reacts with: Human
特異性
Human Galectin 7
免疫原
Recombinant Human Galectin 7/LGALS7 Protein (Catalog#12000-H07E)
調製
Produced in rabbits immunized with purified, recombinant Human Galectin 7/LGALS7 (rh Galectin 7/LGALS7; Catalog#12000-H07E; NP_002298.1; Ser2-Phe136). Galectin 7/LGALS7 specific IgG was purified by Human Galectin 7/LGALS7 affinity chromatography.
ソース
Polyclonal Rabbit IgG
精製
Protein A & Antigen Affinity
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Galectin 7 Antibody (Rabbit Polyclonal 抗体) 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:500-1:1000
IHC-P 1:2500-1:10000
ICC-IF 1:1500-1:50000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Galectin 7 Antibody (Rabbit Polyclonal 抗体) の画像

Anti-LGALS7 rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lane B: 293T Whole Cell Lysate

Lane C: MDA-MB-231 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:15 kDa

Observed band size:15 kDa

(We are unsure as to the identity of these extra bands.)

Immunochemical staining of human LGALS7 in human liver with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).
Immunofluorescence staining of LGALS7 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human LGALS7 polyclonal antibody (dilution ratio 1:5000) at 4℃ overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.

Anti-Galectin 7 Antibody: 別名

Anti-GAL7 Antibody; Anti-LGALS7A Antibody

Galectin 7 背景情報

LGALS7, also known as Galectin-7, is a member of the galectins family. The galectins are a family of beta-galactoside-binding proteins. There are at least 14 identified members in this family. Galectins share similarities in the CRD (the carbohydrate recognition domain). They are synthesized as cytosolic proteins. Though localized principally in the cytoplasm and lacking a classical signal peptide, galectins can also be stimulated to secretion by non-classical pathways or alternatively targeted to the nucleus. Galectins are implicated in modulating cell-cell and cell-matrix interactions. LGALS7 contains 1 galectin domain and is mainly expressed in stratified squamous epithelium. Galectin-7 could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. LGALS7 is a pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release.
完全な名称
lectin, galactoside-binding, soluble, 7
参考文献
  • Villeneuve C, et al. (2011) Mitochondrial proteomic approach reveals galectin-7 as a novel BCL-2 binding protein in human cells. Mol Biol Cell. 22(7):999-1013.
  • Rondanino C, et al. (2011) Galectin-7 modulates the length of the primary cilia and wound repair in polarized kidney epithelial cells. Am J Physiol Renal Physiol. 301(3):F622-33.
  • Masuyer G, et al. (2012) Inhibition mechanism of human galectin-7 by a novel galactose-benzylphosphate inhibitor. FEBS J. 279(2):193-202.
  • Magnaldo T, et al. (1995) Galectin-7, a human 14-kDa S-lectin, specifically expressed in keratinocytes and sensitive to retinoic acid. Dev Biol. 168(2):259-71.
  • Madsen P, et al. (1995) Cloning, expression, and chromosome mapping of human galectin-7. J Biol Chem. 270(11):5823-29.

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