Anti-E2F4 Antibody

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Anti-E2F4 Antibody (Rabbit Polyclonal 抗体) の製品情報

製品名
Anti-E2F4 Antibody
検証済のアプリケーション
WB,ICC/IF
交差反応
Reacts with: Human
Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
特異性
Human E2F4
免疫原
A synthetic peptide corresponding to the center region of the Human E2F4
調製
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human E2F4, and purified by antigen affinity chromatography.
ソース
Polyclonal Rabbit IgG
精製
Protein A & Antigen Affinity
緩衝液
PBS, pH7.0 with 0.03% Proclin300
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.

Anti-E2F4 Antibody (Rabbit Polyclonal 抗体) 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:500-1:2000
ICC-IF 1:100-1:500
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-E2F4 Antibody (Rabbit Polyclonal 抗体) の画像

Anti-E2F4 rabbit polyclonal antibody at 1:500 dilution

Lane A: K562 Whole Cell Lysate

Lane B: Raji Whole Cell Lysate

Lane C: HeLa Whole Cell Lysate

Lane D: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:44 kDa

Observed band size:53 kDa

Immunofluorescence staining of E2F4 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human E2F4 polyclonal antibody (dilution ratio 1:200) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.

Anti-E2F4 Antibody: 別名

Anti-E2F-4 Antibody; Anti-E2F4 Antibody

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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