Anti-CD31 Antibody

Price:
Size:
Number:

Anti-CD31 Antibody (Rabbit Polyclonal 抗体) の製品情報

製品名
Anti-CD31 Antibody
検証済のアプリケーション
WB,ELISA,IHC-P,IP
交差反応
Reacts with: Human
特異性
Human CD31
免疫原
Recombinant Human CD31/PECAM1 Protein (Catalog#10148-H08H)
調製
Produced in rabbits immunized with purified, recombinant Human CD31/PECAM1 (rh CD31/PECAM1; Catalog#10148-H08H; EAW94208.1; Met1-Lys601). CD31/PECAM1 specific IgG was purified by Human CD31/PECAM1 affinity chromatography.
ソース
Polyclonal Rabbit IgG
精製
Protein A & Antigen Affinity
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CD31 Antibody (Rabbit Polyclonal 抗体) 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:500-1:2000
ELISA 1:5000-1:10000
IHC-P 1:1000-1:10000
IP IP:0.5-2μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-CD31 Antibody (Rabbit Polyclonal 抗体) の画像

Anti-CD31 rabbit polyclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:130 kDa

Observed band size:130 kDa

Immunochemical staining of human CD31 in human liver with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of human CD31 in human stomach with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.

CD31 was immunoprecipitated using:

Lane A:0.5 mg Jurkat Whole Cell Lysate

4 µL anti-CD31 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-CD31 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 130 kDa

Observed band size :130 kDa

Anti-CD31 Antibody: 別名

Anti-CD31 Antibody; Anti-CD31/EndoCAM Antibody; Anti-endoCAM Antibody; Anti-GPIIA' Antibody; Anti-PECA1 Antibody; Anti-PECAM-1 Antibody

CD31 背景情報

The Cluster of Differentiation 31 (CD31) adhesion molecule, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1), is the only known member of the CAM family on platelets. CD31 protein is a 13-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, monocytes, neutrophils, and certain T cell subsets. CD31 protein is also expressed in certain tumors, including epithelioid hemangioendothelioma, other vascular tumors, and histiocytic malignancies. CD31 plays a key role in removing aged neutrophils and tissue regeneration. CD31 protein mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin αvβ3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development.
完全な名称
platelet/endothelial cell adhesion molecule 1
参考文献
  • Deaglio S, et al. (2000) CD38/CD31, a receptor/ligand system ruling adhesion and signaling in human leukocytes. Chem Immunol. 75: 99-120.
  • Kohler S, et al. (2009) Life after the thymus: CD31+ and CD31- human naive CD4+ T-cell subsets. Blood. 113(4): 769-74.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

カートに追加しました カートに追加しませんでした カートを更新しています。少々お待ちください