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AARS / alanyl-tRNA synthetase 抗体, ウサギポリクローナル抗体, Antigen Affinity Purified

  • AARS / alanyl-tRNA synthetase Antibody, Rabbit PAb, Antigen Affinity Purified
  • AARS / alanyl-tRNA synthetase Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
  • AARS / alanyl-tRNA synthetase Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
  • AARS / alanyl-tRNA synthetase Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
  • AARS / alanyl-tRNA synthetase Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
データシートレビュー関連製品プロトコル
マウス AARS 抗体製品情報
免疫原:Recombinant Mouse AARS / alanyl-tRNA synthetase Protein (Catalog#50384-M08B)
Clone ID:
Ig タイプ:Rabbit IgG
濃度:
エンドトキシン:
製剤:0.2 μm filtered solution in PBS
調製:Produced in rabbits immunized with purified, recombinant Mouse AARS / alanyl-tRNA synthetase (rM AARS / alanyl-tRNA synthetase; Catalog#50384-M08B; Q8BGQ7; Met1-Asn968). AARS / alanyl-tRNA synthetase specific IgG was purified by Mouse AARS / alanyl-tRNA synthetase affinity chromatography.
マウス AARS Antibody WB Application Image
マウス AARS Antibody IHC Application Image
AARS / alanyl-tRNA synthetase Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
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Caption:
Immunochemical staining of mouse AARS in mouse kidney with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
AARS / alanyl-tRNA synthetase Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
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Caption:
Immunochemical staining of mouse AARS in mouse stomach with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
マウス AARS Antibody IF Application Image
AARS / alanyl-tRNA synthetase Antibody, Rabbit PAb, Antigen Affinity Purified
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Caption:
Immunofluorescence staining of AARS in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-mouse AARS polyclonal antibody (1 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
マウス AARS Antibody IP Application Image
Other AARS Antibody Products

Anti-Histone H3 rabbit monoclonal antibody at 1:200 dilution

Lane A: NIH3T3 Whole Cell Lysate

Lane B: Hela Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:15 kDa

Observed band size:17 kDa

AARS / alanyl-tRNA synthetase 背景

Alanyl-tRNA synthetase (AARS) belongs to the family of ligases, specifically those forming carbon-oxygen bonds in aminoacyl-tRNA and related compounds. This enzyme participates in alanine and aspartate metabolism and aminoacyl-tRNA biosynthesis. Alanyl-tRNA synthetase (AlaRS) catalyzes synthesis of Ala-tRNA (Ala) and hydrolysis of mis-acylated Ser- and Gly-tRNA (Ala) at 2 different catalytic sites. Their role is not confined to catalyze the attachment of amino acids to transfer RNAs and thereby establish the rules of genetic code by virtue of matching the nucleotide triplet of anticodon with cognate amino acid. Under apoptotic conditions in cell culture, the full-length enzyme is secreted, and the two cytokine activities can be generated by leukocyte elastase, an extracellular protease. Secretion of this tRNA synthetase may contribute to apoptosis both by arresting translation and producing needed cytokines. This protein could be an attractive target of drugs against bacterial, fungal and parasitic infections. 

マウス AARS / alanyl-tRNA synthetase 参考文献
  • Wakasugi K, et al. (1999) Two Distinct Cytokines Released from a Human Aminoacyl-tRNA Synthetase. Science. 284 (5411): 147-51.
  • Sokabe M, et al. (2009) The structure of alanyl-tRNA synthetase with editing domain. Proc Natl Acad Sci . 106 (27): 11028-33.
  • Skupinska M, et al. (2009) AARS--the etiological factor and the attractive target of many disorders. Postepy Biochem. 55 (4): 373-84.
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    カタログ: 50384-T60-200
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    Datasheet & Documentation

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